Takara Shuzo Co., Inc.

Takara Bio Inc.

Other

Johns Hopkins University

Kiyozo Asada

Ikunoshin Kato

Takashi Uemori

Hiroyuki Mukai

Nobuto Koyama

Hikaru Takakura

Kimikazu Hashino

Mio Morishita

Takashi Ueno

Yoshimi Sato

Kazue Miyake

Tomoko Fujita

Osamu Takeda

Tomoko Shimojo

Junko Yamamoto

Masanori Mitta

Susumu Tsunasawa

Katsuhiko Yamamoto

Toshiharu Ohba

Masanori Takayama

Kazuhiko Nishitani

Shuichi Takahashi

Kazuhide Okazawa

Hiroaki Sagawa

Hideto Chono

Hirokazu Kotani

Michio Hagiya

Mitsuhiro Ueno

Masamitsu Shimada

Kenji Sobue

Kei Fujinaga

Kazuyori Ochiai

Takashi Hashida

Keiko Kubo

Yoshiko Anma

Ken'ichiro Hayashi

Eiji Kobayashi

Tatsuji Enoki

Junichi Mineno

Jun Tomono

Fusao Kimizuka

Hideyo Yamaguchi

Mutsumi Sano

Hirofumi Yoshioka

Keiji Tanaka

Fumitsugu Hino

Masahiro Endo

Tsuyoshi Miyamura

Yoshie Yoshikawa

Christian B Anfinsen

Hiroshi Kobori

Hiromi Okuyama

Yasushi Katayama

Ryo Hirano

Yuji Hashimoto

Kenneth Laderman

Tomoo Inden

Tooru Suzuki

Yuko Nakabayashi

Satoru Fukuda

Naotsune Saga

Sawako Nagashima

Ikunoshi Kato

Hajime Matsumura

Tomoko Iwasaki

Mariko Moriyama

Haruhisa Sawaragi

Toshitake Okui

Haruko Konishi

Mariko Okawa

Kahoko Umeda

Kimakazu Hasino

Miki Odate

Atsushi Shimada

The present invention pertains to a method for modifying a nucleic acid contained in a sample comprising a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, comprising the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

Method for modifying nucleic acids

The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40&#xb0; C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3' or 5&#x2032; end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.

Method for producing RNA-containing probe for detecting a target nucleotide

A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

Method for synthesizing cDNA

A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.

Method of retrovirus storage

A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3'&#x2192;5&#x2032; exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3&#x2032;&#x2192;5&#x2032; exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3&#x2032;&#x2192;5&#x2032; exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.

Method for synthesizing DNA

A polypeptide having a endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.

Endoribonuclease

A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.

An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.

Enhancement of retroviral gene transduction employing polypeptides comprising the fibronectin heparin II binding domain

Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.

Promoters

A promoter, an intron and a terminator by which a useful foreign gene can be efficiently expressed in algae and which are thus useful in breeding algae using genetic engineering techniques.

Alga-origin promoter, intron and terminator

A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.

Method for gene transfer into target cells with retrovirus

Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.

Thermotolerant ribonuclease H

There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Hyperthermostable protease gene

Growing community of inventors

Shiga, Japan

Kiyozo Asada