Mitsui Chemicals, Inc.

Kiyoteru Nagahara

Tadashi Araki

Takeshi Nakamura

Tomonori Hidesaki

Yasushi Fukuiri

Mitsuo Koito

Keita Nishida

Hiroki Ishibashi

Toshihiro Oikawa

Kiyoshi Ito

Tamotsu Asano

Seiichi Watanabe

Yasuko Matsuba

Kaori Matoishi

Ichirou Ikeda

Reiko Abe

Tomoyuki Kawabata

Katsuyuki Takahashi

This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

DNA encoding novel enzyme having D-serine synthase activity, method of producing the enzyme and method of producing D-serine by using the same

Provided is a purified lactic acid-glycolic acid copolymer, preferably in the form of powder, having a reduced content of dimers such as residual lactide and glycolide that can be produced by a simple industrial process without the use of any high-shear special device. Further provided is a high-purity lactic acid-glycolic acid copolymer which has small amounts of dimers such as residual lactide and glycolide and small amounts of a low-molecular weight lactic acid-glycolic acid copolymer or a salt thereof and which has a small ratio of weight average molecular weight to number average molecular weight (Mw/Mn). The copolymer can be produced in accordance with a defined process.

Process for producing a lactic acid-glycolic acid copolymer or a salt thereof

This invention relates to DNA encoding an enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

The invention provides a method for producing a cytosine nucleoside compound from pentose-1-phosphate and cytosine or a derivative thereof using a nucleoside phosphorylase reactive to cytosine or a bacterium having the enzyme activity. The invention also provides a method for specifically reducing an activity to degrade the substrates or the product, resulting in efficient production of the cytosine nucleoside compound. According to the invention, little by-product is produced in producing cytonucleocide compounds.

Method for producing cytosine nucleoside compounds

A protected 2'-deoxycytidine is purified by precipitating the protected 2&#x2032;-deoxycytidine represented by general formula (3) in the form of a hydrated crystal from a solution containing the protected 2&#x2032;-deoxycytidine and water, and by recovering the protected 2&#x2032;-deoxycytidine:wherein R1 represents a 4-methoxytrityl, 4, 4&#x2032;-dimethoxytrityl, or triphenylmethyl group; and B1 represents a cytosine group having a protected amino group. The compound represented by general formula (3) is, in particular, a protected 2&#x2032;-deoxycytidine represented by formula (4):The 2&#x2032;-deoxycytidine is used as a raw material for antisense DNA.

Method for purifying protected 2'-deoxycytidines and hydrated crystals thereof

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Omuta, Japan

Kiyoteru Nagahara