Riken Corporation

Kiyoshi Ohyama

Kazuki Saito

Toshiya Muranaka

Hikaru Seki

Hiroshi Sudo

Satoru Sawai

Provided are an enzyme involved in a glycyrrhizin biosynthetic system, a gene of the enzyme and use thereof in order to stably and continuously provide a large amount of glycyrrhizin. Glucuronosyltransferase with an activity of further transferring glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide is identified to provide the transferase, a gene for the transferase and use thereof.

Glucuronosyltransferase, gene encoding same and use thereof

Identification of a protein having an activity of oxidizing oleanane-type triterpene, and a gene encoding the protein, the protein and the gene, and use thereof are provided. For example, a protein having an activity of oxidizing oleanane-type triterpene obtained from a plant in the family Fabaceae, a gene encoding the protein and use thereof are provided. The protein is shown in, for example, SEQ ID NO: 4, 14 or 18, and the gene encoding the protein is shown in, for example, SEQ ID NO: 3, 13 or 17. A transformant into which the gene is introduced can be produced, and thereby a triterpene oxidase can be obtained.

[object Object]

Provided is a protein having an activity of oxidizing a dammarane-type triterpene, a gene encoding the protein, and a method of using the protein and the gene. The protein can be obtained from a plant belonging to the genus, which has an activity of oxidizing a dammarane-type triterpene. Also provided is a transformant producing a triterpene oxidase into which a gene encoding the triterpene oxidase is introduced.

Growing community of inventors

Kanagawa, Japan

Kiyoshi Ohyama