Mitsui Chemicals, Inc.

Komori Printing Machinery Co., Ltd.

Kiyoshi Ito

Takeshi Nakamura

Toshifumi Yamaki

Teruo Arii

Miyuki Tsuruoka

Tamotsu Asano

Takeya Abe

Takeshi Fukuda

Hideki Kobayashi

Nobuyuki Fukazawa

Tomoyuki Ando

Shinichi Banba

Tadashi Araki

Hironori Komatsu

Katsutoshi Tsuchiya

Toshihiro Oikawa

Eishi Tanaka

Seiichi Watanabe

Hiroki Ishibashi

Yasushi Fukuiri

Kaori Matoishi

Hitoki Miyake

Hideki Umetani

Tadashi Suzuki

Kenju Sasaki

Ichirou Ikeda

Hiroko Shibamoto

Tamotsu Omori

Junya Fujiwara

Seiya Nikumaru

Masanori Muramoto

Toshihisa Tachibana

Takahiro Yamauchi

Kyoko Maruyama

Hirokazu Awano

Toshikazu Aikawa

Souichi Hazama

Katsuyuki Takahashi

A method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity wherein the concentration of benzene in the aqueous medium is 4.0 ppm or less and a method for producing an amide-based polymer excellent in quality from the amide compound. Also provided are a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between water solubility and high molecular weight and is excellent in quality.

Method for producing amide compound

The amino acid sequence of a mutant which is obtained by introducing a novel mutation into aJCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.

Nitrile hydratase variant

A desired isomer is selectively prepared by phosphorolyzing and isomerizing an anomer mixture of a 1-phosphorylated saccharide derivative while crystallizing one of the isomers to displace the equilibrium. Furthermore, using the action of a nucleoside phosphorylase, a nucleoside is prepared from the 1-phosphorylated saccharide derivative obtained and a base with improved stereoselectivity and a higher yield. This process is an anomer-selective process for preparing a 1-phosphorylated saccharide derivative and a nucleoside.

Process for selectively producing 1-phosphorylated sugar derivative anomer and process for producing nucleoside

The invention is a process for continuously producing an amide compound by reacting a microorganism fungus body containing nitrile hydratase or a processed product of the microorganism fungus body with a nitrile compound in an aqueous medium, characterized in that after contacting said fungus body or said processed product of said fungus body with said nitrile compound in said aqueous medium, a reaction solution thus obtained is further subjected to reaction under conditions having a plug flow region, and according to the invention, an amide compound aqueous solution of a high concentration a high purity can be easily obtained in an extremely high conversion rate of a nitrile compound without a condensation process.

Process for producing amide compounds

The invention provides a nitrile hydratase activation protein participating in activation of nitrile hydratase derived from JCM3095, and a gene sequence encoding the same. Further, the invention provides a recombinant plasmid containing the gene, a recombinant plasmid containing the gene and a nitrile hydratase gene, a transformant strain obtained through transformation with the recombinant plasmid, and a process for producing a corresponding amide compound from a nitrile compound using a culture solution and cells obtained by incubating the transformant strain and treated products of the cells.

Protein participating in the activation of nitrile hydratase and gene encoding the same

The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.

Nitrile hydratase

In apparatus for supplying ink and or water to a plate cylinder through fountain roller, swinging roller and a transfer roller, the transfer roller is supported by a swinging lever and the motion thereof is allowed or prevented by a spring biased control member. Such allowance and prevention is selected by an operating handle.

Transfer roller switching mechanisms of ink and water supply apparatus

In a varnish coater, a set of a blanket cylinder and a form roller and a set of a pan roller and a metering roller are driven by different drive sources. One-way clutches are arranged between the blanket cylinder and the form roller and between the form roller and a motor as one of the different drive sources, respectively. The form roller is selectively driven by one of the different drive sources through a corresponding one-way clutch. Alternatively, the form roller is driven by one of the drive sources which has a higher rotational speed.

Varnish coater for printed product

In a varnish coater for a printed product, a blanket cylinder and a form roller are respectively supported by eccentric bearings to throw on/off the blanket cylinder with respect to the form roller and an impression cylinder and throw on/off the form roller with respect to the blanket cylinder, and rollers provided in the eccentric bearings of the form roller are brought by biasing means into tight contact with the cam surfaces of cams pivoted by pivot means so as to simplify adjustment of a contact pressure of the form roller with respect to the blanket cylinder at the throw-on and -off positions of the blanket cylinder.

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Chiba, Japan

Kiyoshi Ito