Director of National Food Research Institute, Ministry of Agriculture,

Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

Bio-oriented Technology Research Advancement Institution

National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

Bio-oriented Technology Research Advanced Institution

Kiyoshi Hayashi

Yutaka Mori

Ken Tokuyasu

Shioka Hamamatsu

Hiroshi Ono

Sachiko Machida

Mayumi Kameyama

Kazutomo Haraguchi

Takeshi Takaha

Kazutoshi Fujii

Yoshinobu Terada

Satoru Nirasawa

Yuki Kitagawa

Satoshi Kaneko

Tsuyoshi Shimonishi

Hebiao Li

Aimin Liu

Yoshiaki Kitamura

The present invention provides an artificial chaperon useful for refolding the proteins having low voluntary folding ability and being difficult or unable to be a native form without a second (or assistant) of a molecular chaperon in a short time, and folding said proteins as an active form. The present invention relates to an artificial chaperon kit characterized in that the kit comprises cyclic saccharide cycloamylose and polyoxyethylenic detergent or cyclic saccharide cycloamylose and ionic detergent. The present invention also relates to a method for diluting the denaturant making the protein a denatured state by adding a specific detergent to a denatured protein, and preventing protein molecules from aggregation, thereafter adding cyclic saccharide cycloamylose, utilizing the inclusion ability thereof to strip detergent, accelerating the proper folding of protein into a correct higher-order structure with activity.

Artificial chaperon kit

A method for activating a denatured protein, includes the steps of: adding a detergent to the denatured protein to allow a protein-detergent complex to be formed; and adding high-molecular weight amylose to the protein-detergent complex so that the high-molecular weight amylose removes the detergent.

Method for activating denatured protein

2-methyl-(4-O-(2-amino-2-deoxy-&bgr;-glucopyranosyl)-1,2-dideoxy-&agr;-glucopyrano&rcub;(2,1-D)-2-oxazoline and its salt, 50% deacetylated chitin or its oligosaccharide and salt thereof

A chitin deacetylase gene encoding a protein, a plasmid vector containing said gene and a transformant transformed with the plasmid vector. An object of the present invention is to make contribution to the industrial production of said enzyme, by cloning the gene of said enzyme to elucidate the structure of the gene and express the gene.

Chitin deacetylase gene, vector containing said gene and transformant

The present invention relates to polynucleotides encoding particular N-acetylmuramidases, which are cell wall lytic enzymes, of Streptomyces rutgersensis origin. The invention also relates to vectors comprising the polynucleotides encoding the N-acetylmuramidases, and also relates to host cells transformed with the vectors.

Gene of cell wall lytic enzyme, and vector containing said gene and

Disclosed is p-nitrophenyl 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-.beta. -D-glucopyranoside of formula (1) and its acid-addition salts. ##STR1## The compound of formula (1) is produced by reacting a p-nitrophenyl derivative of chitin dimer with a microorganism-derived deacetylase. The compound and its salts do not act on exo-type chitinases, and are specific to only endo-type ones.

p-Nitrophenyl

Di-N-acetyl-D-chitobiose, which is easily available as a chitin decomposate or a chemical reagent, is reacted with a microorganisms-derived deacetylase to produce 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-D-gluc ose of formula (1). ##STR1## The method is quantitative and efficiently produces the compound (1). The compound (1) and its salts are usable as substrates for measuring enzymatic activities, and also as starting substances in the production of various food materials and in the field of glyco-chain engineering.

Method for producing

Disclosed is a cellobiose phosphorylase gene coding for a protein consisting of the amino acid sequence of SEQ ID NO: 16 as set forth in the Sequence Listing, a plasmid vector comprising the cellobiose phosphorylase gene and a transformant transformed with the plasmid vector.

Cellobiose phosphorylase gene, vector and transformant containing said

Growing community of inventors

Tsuchiura, Japan

Kiyoshi Hayashi