Sapporo Medical University

Johnson & Johnson Kabushiki Kaisha

Kiyohiro Houkin

Osamu Honmou

Tomohiko Asahara

Takeshi Mikami

The present invention relates to a method for growing, rapidly and massively ex vivo, cells collected from a living subject to provide a safe and effective pharmaceutical preparation for biological tissue repair/regeneration. Specifically, the present invention relates to a method for growing cells in a sample collected from a living subject by culturing the cells in a medium containing allogeneic (including autogenic) serum. Preferably the allogeneic serum has been determined as being negative for a serum tumor marker and/or an infectious factors, and the amount of the anticoagulant (e.g., heparin, a heparin derivative, or a salt thereof) added to the collected sample is less than 5 U/mL with respect to the volume of the sample or the amount of the anticoagulant in the medium at the start of culture is less than 0.5 U/mL. The present invention further relates to use of the method.

Cell growth method and pharmaceutical preparation for tissue repair and regeneration

This invention provides bipolar forceps that are used for coagulation, dissection or similar surgical manipulation by the application of a high-frequency current in a surgical operation, which suppresses attachment and sticking of burned protein to arm tip end portions irrespective of the properties of the metal material forming the arms. The bipolar forceps according to the present invention have on each of opposed surfacesof the tip end portionsof a pair of arms a composite plating filmcomposed of a noble metal materialand nonconductive fine particles, and a laminate plating filmof a noble metal material formed on the composite plating film

Growing community of inventors

Hokkaido, Japan

Kiyohiro Houkin