Bigtec Private Limited

Kirubakaran Naveen Kumar

Chandrasekhar Bhaskaran Nair

Pillarisetti Venkata Subbarao

Mulakkapurath Narayanan Manoj

Venkata Ramachandra Rao Vasamsetty

Madhuri Baliga

In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.

Method of downstream processing for conversion of human insulin precursor molecule into a functional human insulin protein molecule

Polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

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Bengaluru, India

Kirubakaran Naveen Kumar