Dsm IP Assets B.v.

Evonik Operations Gmbh

Kirt Lyvell Matthews, Sr

Neil Francis Leininger

Ginger Marie Shank

Nasrin Tabayehnejad

Michael B Johnson

Mark Barker

Vinod Tarwade

Christian Rabe

Martin Heining

Holger Pfeifer

Jochen Lebert

Xiao Daniel Dong

David Allen Tinsley

Shannon Elizabeth Ethier Resop

Michael Diehl

Georg Borchers

John Hogan

Stephen Cherinko

Manfred Bärz

Micah Needham

Robert Cody Kertis

Marc Beiser

Annika Hartmann

Negash Adugna

Mathias Dernedde

Daniel Verkoeijen

Jürgen Haberland

Mark Edward Nejako, Ii

Brock Triplett

Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Method of reducing emulsion by broth washing

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

Method of separating lipids from a lysed lipids containing biomass

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

Method of isolating lipids from a lipids containing biomass

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Processes for obtaining microbial oil from microbial cells

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PU-FAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Processes are disclosed for obtaining a lipid from a cell by lysing the cell to form a lysed cell composition, adding an acid to lower the pH of the lysed cell composition, and demulsify the lysed cell composition, and obtaining the lipid from the demulsified lysed cell composition. Also disclosed are lipids prepared by the processes of the present invention wherein the lipids have a particular anisidine value, peroxide value, and/or phosphorus content.

Growing community of inventors

Fort Mill, SC, United States of America

Kirt Lyvell Matthews, Sr