The Charles Stark Draper Laboratory, Inc.

Kirsty A McFarland

Andrew P Magyar

Peter Cavanagh

Jason Wyatt Holder

Melissa M Sprachman

Miles Rogers

Erin Rosenberger

Nicole E Raustad

Gregg E Favalora

Steven J Byrnes

Connor McBrine

Ian Ward Frank

Kenneth T Kotz

Charles A Lissandrello

Sarah Gruszka

Ryan A Dubay

Michael Springer

Ting Pang

Amanda Nicole Billings-Siuti

Stacey Markovic

Haiyao Huang

Emma Vargo

Helen Bartlett

Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3' end of a growing single-stranded DNA polynucleotide.

Modified template-independent DNA polymerase

A parallelized chain-synthesizing technique includes capillary tubes, where each tube provides multiple locations or addresses where a specific arbitrary sequence for polymeric chains can be synthesized. An optical addressing system selectively delivers light to the locations to mediate or control reactions in the tubes.

Apparatus for high density information storage in molecular chains

The present disclosure provides methods of generating recombinant bacteriophage genomes via semi-synthesis. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Bacteriophage engineering via semi-synthesis

Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.

Miniaturized DNA microarray for small-volume sample processing

Methods of detecting unknown genetic modifications in a DNA sample from an organism are disclosed.

Hybridization immunoprecipitation sequencing (HIP-SEQ)

The present disclosure provides compositions including recombinant K or 812 bacteriophages, methods for making the same, and uses thereof. The recombinant K or 812 bacteriophages disclosed herein are useful for the identification and/or antibiotic susceptibility profiling of specific bacterial strains/species present in a sample.

Recombinant K and 812 bacteriophages and uses thereof

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Boston, MA, United States of America

Kirsty A McFarland