Amaxa Ag

Lonza Cologne Gmbh

Lonza Cologne Ag

Kirsten Rothmann

Herbert Mueller-Hartmann

Ludger Altrogge

Rainer Christine

Heike Wessendorf

Gregor Siebenkotten

Gudula Riemen

Elke Lorbach

Juliana Helfrich

Herbert Müller-Hartmann

Helmut Brosterhus

Corinna Thiel

Meike Weigel

Michael Nix

Dietmar Lenz

Marion Gremse

Helmut Brosterbus

Sabine Poppenborg

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol&#xd7;pHand an ionic strength of at least 200 mmol&#xd7;1during a change to the pH value from pH 7 to pH 8 and at a temperature of 25&#xb0; C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.

Buffer solution for electroporation and a method comprising the use of the same

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Kö'ln, Germany

Kirsten Rothmann