Teknologian Tutkimuskeskus Vtt Oy

Kirsi Bromann

Laura Ruohonen

Mervi Toivari

Merja Elisa Penttila

Tiina Nakari-Setälä

Tina Nakari-Setala

Merja PenttilÄ

Tiina Nakari-SETÄLÄ

The present invention concerns a method for producing terpenes in fungi comprising the steps of (a) providing a modified terpene biosynthetic gene cluster inside a host cell, wherein one or more of the naturally occurring genes or promoters of the cluster have been replaced, truncated or removed, (b) providing a transcription factor inside the host cell, the transcription factor activating the terpene biosynthetic gene cluster; (c) cultivating said host in conditions allowing the expression of the transcription factor activating the cluster; and optionally (d) recovering the thus produced terpene product.

Method for producing terpenes

The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use ofFGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.

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Espoo, Finland

Kirsi Bromann