Genencor International, Inc.

Genentech, Inc.

Akzo Nobel N.v.

Carebay Holding Ltd.

Itero Biopharmaceuticals, Inc.

Other

Henry G. Heinsohn

Jeffrey D. Lorch

Kirk J. Hayenga

Raymond E. Arnold

Carebay Holding Ltd., Company No. 681498

Kirk James Hayenga

Virgil B Lawlis

Randy M Berka

Gregory Lawrence Gray

Daniel Cullen

Michael C Ward

Lucio Giambattista

Antonio A Bendek

Jeffrey D Lorch

Henry G Heinsohn

Raymond E Arnold

David Desalvo

Darlene P Horton

V Bryan Lawlis

Richard L Vandlen

Daniel G Yansura

Bradley Richard Snedecor

Ernst H Rinderknecht

Terry Hauser

Pascal P Valex

Tim Breece

A one-time use device to deliver preservative-free follicle stimulating hormone (FSH) solution is disclosed. The device includes a needle covered by a sliding needle shield, which covers the needle in all modes of the device. The device can be placed into a ready-to-use position in four or fewer user steps. The device has a knob for setting a desired dose of FSH. The knob includes longitudinally spaced elements respectively corresponding to the lock position and the seven or fewer discrete dosing positions. The device locks after one use and cannot be reused thereafter.

Preservative-free follicle stimulating hormone solution delivery device

The current invention provides methods for molecule purification by RP-LC and RP-HPLC that uses unbranched terminal alkyldiols as eluting solvents. In particular, the present invention purifies molecules, particularly proteins and peptides, on reverse phase liquid chromatography columns using a buffer containing either 1,5 pentanediol, 1,6 hexanediol or 1,7 heptanediol.

Method of purification of molecules using unbranched terminal alkyldiols

This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.

Aspartic proteinase deficient filamentous fungi

Methods are provided in this invention for the isolation of human growth hormone, growth hormone antagonist, or a homologue of either, from a biological source. The methods of the invention use multi-phase extraction.

Methods for protein purification using aqueous two-phase extraction

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3&prime; terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same

Heterologous polypeptides expressed in filamentous fungi, process for making same, and vectors for making same

Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

Heterologous polypeptides expressed in filamentous fungi, processes for

A process for the prokaryotic production of relaxin from non-naturally occurring forms of prorelaxin is provided. Also provided are non-naturally occurring forms of prorelaxin.

Process for producing relaxin

Heterologous polypeptides expressed in aspergillus

Disclosed are methods for the recovery and purification of naturally produced chymosin. In particular, disclosed are methods for the recovery and purification of chymosin from aqueous solutions containing chymosin, pepsin and other contaminants.

Processes for the recovery of naturally produced chymosin

Disclosed are methods for the recovery of microbially produced chymosin. In particular, disclosed are methods for the recovery of chymosin from the fermentation beer arising from culturing microorganisms which have been engineered so as to produce chymosin. Also disclosed are methods for the selective recovery and subsequent purification of microbially produced chymosin.

Processes for the recovery of microbially produced chymosin

Growing community of inventors

Burlingame, CA, United States of America

Kirk James Hayenga