Thorlabs Gmbh

Kirk Gossage

Alex Cable

Jeffrey S Brooker

James Jiang

Dierck Hillmann

Jason Matthew Mills

Jörn Wollenzin

Ross Johnstone

Jeffrey Erickson

Jorn Wollenzin

Jason Mills

Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

Microscopy system with auto-focus adjustment by low-coherence interferometry

A method for correcting images acquired via an asynchronous triggered acquisition, wherein image acquisition is triggered by a trigger signal based on the motion and/or position of the sample to be imaged by an image sensor, the method including: measurement of the motion and/or position of the sample; determination of an exposure time based on the aforementioned measurement; and correction of an acquired image based on the determined exposure time and a desired exposure time.

Method for correcting images acquired via asynchronously triggered acquisition

A method for matching one or more channels to a reference channel in a multiple-channel detector having a digital output, the method including: receiving an analog-to-digital unit (ADU) input value from the multiple-channel detector for each channel; and replacing the ADU input value by an output value corresponding to each channel from a look up table (LUT); wherein the LUT is created by taking a relative intensity ratio of signals from each channel compared to the reference channel over a range of voltage values; calculating a signal value on the detector that generates a specific ADU value across the entire ADU range of the detector; interpolating the relative intensity ratios to obtain ratios corresponding to the calculated signal values for each channel; and dividing the each of the specific ADU value by the corresponding interpolated ratio to obtain the corresponding output value for each channel.

Multiple channel matching method

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Austin, TX, United States of America

Kirk Gossage