Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Kirill Kolmakov

Stefan W Hell

Christian Wurm

Vladimir Belov

Lars Kastrup

Gerald Donnert

Katrin Willig

Shamil Nizamov

Heiko Schill

Johanna Wildanger

Volker Westphal

Christian Eggeling

Stefan Jakobs

Marcel Lauterbach

Christian Ringemann

Christian A Wurm

The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O)YYor P(O)YY, where Yand Yindependently denote any of the following residues: OH, O(&#x2212;), ORand OR, NHRand NHR, NRRand NRR, ORand NHR, ORand NRR, NHRand NRR; and any salt thereof.

Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques

The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH, C&#x2550;O, C&#x2550;NOR, C&#x2550;NNRNR, CH(OR), O, S, SO, SO, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion. 

Hydrophilic and lipophilic rhodamines for labelling and imaging

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St. Petersburg, Russia

Kirill Kolmakov