The Samuel Roberts Noble Foundation

Purdue Research Foundation

Other

Kirankumar S Mysore

Stanton B Gelvin

Richard A Dixon

Srinivasa Rao Uppalapati

Lloyd W Sumner

Wensheng Li

Kan Wang

Zarir E Vaghchhipawala

Ajith Anand

Choong-Min Ryu

Bronwyn R Frame

Million Tadege

The invention provides methods and compositions for making a dicotyledonous plant that is susceptible toRoot Rot (PRR) more resistant to PRR, by metabolic engineering of the plant's flavonoid and isoflavonoid biosynthetic pathways. Thus, methods for increasing the synthesis and accumulation of medicarpin and/or 7,4'-dihydroxyflavone in plants such as alfalfa are provided.

Metabolic engineering for plant disease resistance

The invention provides coding and promoter sequences for an STF gene, which affects lamina expansion in plants. Vectors, transgenic plants, seeds, and host cells comprising a heterologous STF gene are also provided. Additionally provided are methods of altering biomass in a plant using the STF gene.

Methods and compositions for altering plant biomass

The invention provides methods and compositions for increasing the efficiency of genetic transformation of host cells, including plant cells, and other eukaryotic cells, by reducing the expression of a polypeptide active in a pathway, such as the NHEJ pathway, for repairing damage to the cellular genome. In certain embodiments, the polypeptide is active in repairing double strand breaks (DSB's) of a cellular genome, and may include XRCC4, KU70, KU80, the DNA-activated Protein Kinase (DNA-Pkcs), and ATM. Methods for enhancing the resistance of plant cells to Crown Gall disease are also provided. In another aspect, genetic regulatory elements are provided, including an XRCC4 promoter.

Methods and compositions for increasing plant transformation efficiency

The invention provides transgenic plants with resistance to infection by a root-infecting fungal plant pathogen such as. Also provided are methods of making such plants. Further provided are nucleic acid vectors for producing such a plant. Additionally, methods are provided for growing a dicotyledonous plant that is resistant to root rot disease in soil that comprises, or another pathogen.

Disease resistant plants

The invention provides methods and compositions for enhancing the efficiency of-mediated transformation of host cells such as plant cells. Plant expression constructs comprising a gene encoding a VIP2 or VIP2-like polypeptide are provided, as well as methods for utilizing such constructs to enhance-mediated transformation efficiency.

Method for agrobacterium-mediated transformation of plants

The invention provides novel methods and compositions for modulating gene function in plants. In particular, the invention provides methods and compositions that allow efficient induction of virus-induced gene silencing in plants. The invention is significant in that it allows high throughput analysis of gene function in plants.

Root agroinoculation method for virus induced gene silencing

Methods and compositions to increasetransformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.

Methods and compositions for enhanced plant cell transformation

Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by. The histone H2A gene encoded by theRAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration.genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable byand transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.

Enhanced plant cell transformation by addition of host genes involved in T-DNA integration

Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration. genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable by Agrobacterium and transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.

Growing community of inventors

Ardmore, OK, United States of America

Kirankumar S Mysore