Tohoku University

The University of Tokyo

Kumiai Chemical Industry Co., Ltd.

Kinya Toriyama

Kiyoshi Kawai

Koichiro Kaku

Tsutomu Shimizu

Kenta Katayama

Shin-ichi Arimura

Tomomi Hidaka

Ayako Okuzaki

Tomohiko Kazama

Nobuhiro Tsutsumi

Sota Fujii

A method for inducing a structural change in a plant mitochondrial genome is provided. The invention relates to a method for inducing a structural change in a mitochondrial genome in a plant cell by introducing a double-strand break into a target sequence region on mitochondrial genomic DNA in a plant cell, which is present in individual molecule species of the mitochondrial genomic DNA. In addition, the present invention also relates to a method for deleting a gene that is present in mitochondrial genomic DNA in a plant cell by introducing a double-strand break into the gene or a region near the gene, which is present in individual molecule species of the mitochondrial genomic DNA. Moreover, the invention also relates to a plant cell having a mitochondrial genome, in which a structural change has been induced by the method, and a seed and a plant having the plant cell.

Method for editing plant mitochondrial genome

Mainly provided is a technique for directly identifying the genotype at locus Rf17 based on the specific base sequence data thereof. Also provided is a technique for artificially constructing a fertility-restored line. A method of restoring the fertility of CW-type cytoplasmic male sterile rice by inhibiting or reducing the expression of a gene comprising the base sequence represented by SEQ ID NO:2 in the above-described rice, and a method for determining the presence or absence of gene Rf17, which is a fertility restorer gene for CW-type cytoplasmic male sterility, comprising identifying a single nucleotide polymorphism (SNP) in the base at the 1812 position of the base sequence represented by SEQ ID NO:1 in the rice to be examined.

Fertility restorer gene and fertility restoration method for CW-type male sterile cytoplasm of rice

Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.

Method for transformation using mutant acetolactate synthase gene

Growing community of inventors

Sendai, Japan

Kinya Toriyama