Mect Corporation

Kanto Ishi Pharmaceutical Co., Ltd.

Kinji Takada

Masayoshi Ito

Yoshiyasu Shitori

Yoshitaka Nagai

Hideki Yamamoto

Shoji Yoshimura

Isao Suda

Hiroyuki Takano

Shigeo Shimizu

Atsushi Kawamura

A hybridoma producing a monoclonal antibody specific for free N-acetylneuraminic acid or beta glycoconjugates thereof, is herein disclosed. The hybridoma can be generated by fusing (i) B cells of lymphocytes obtained by immunizing an animal with an N-acetylneuraminic acid or a beta-glycoconjugate thereof, and (ii) myeloma cells.

Monoclonal antibodies specific for free N-acetylfuraminic acid and

A monoclonal antibody specifically recognizes an epitope or an antigenic determinant represented by the following formula and specifically reacts therewith: ##STR1## The monoclonal antibody can be obtained by cultivating hybridoma cells which can be generated by a method comprising cell-fusing (A) B cells or lymphocytes obtained by immunizing an animal with a substance involving the epitope or the antigenic determinant represented by the foregoing formula and (B) myeloma cells. The monoclonal antibody shows high specificity to ganglioside GQ.sub.1b and ganglioside GT.sub.1a having a specific epitope and thus can detect the epitope in high sensitivity. Therefore, the antibody can be used for the elucidation of sugar chain's roles in cell functions and for studying the development of animals. Moreover, the monoclonal antibody would have various clinical applications.

Monoclonal antibodies capable of recognizing gangliosides GQ.sub.1b and

A hybridoma capable of producing a monoclonal antibody specific to a sialic acid containing glycolipid carrying N-acetylneuraminic acid residue having alpha 2.fwdarw.3 linkage is herein disclosed. The hybridoma can be generated by fusing (i) B cells or lymphocytes obtained by immunizing an animal with a sialic acid containing glycolipid carrying N-acetylneuraminic acid residue having alpha 2.fwdarw.3 linkage and (ii) myeloma cells. The monoclonal antibody produced by the hybridoma can be used for purifying gangliosides, for treating patients suffering from melanoma and for diagnosing sera.

Monoclonal antibody recognizing alpha 2.fwdarw.3 bonds

Novel cephalosporin derivatives are described which have excellent antibiotic activity against pathogenic bacteria belonging to the Pseudomonas, Serratia and Enterobacter. The compounds have general formula ##STR1## in which R is hydrogen, acyloxy, carbamoyloxy, a substituted or unsubstituted pyridinium group or a group -S-Het in which Het represents a substituted or unsubstituted, hetero atom-containing, 5- or 6-membered heterocyclic ring, R' is hydrogen, alkali metal, an organic amine or an ester moiety, R.sub.1 and R.sub.2 are the same or different and are hydrogen or a lower alkyl and B stands for a 1,4-cyclohexadienyl group, a group ##STR2## in which Y is hydrogen, --OH or ##STR3## in which R.sub.5 is an alkyl of 1 to 5 carbon atoms, aryl or alkoxy having 1 to 4 carbon atoms, Z stands for hydrogen or halogen and p is an integer of 1 or 2, a furan group or a thiophene group. Methods of preparation are described.

Cephalosporins

Growing community of inventors

Tokyo, Japan

Kinji Takada