Miles Inc.

Bayer Corporation

Ascensia Diabetes Care Holdings Ag

Bayer Healthcare LLC

Miles Laboratories, Inc.

Kin-Fai Yip

Huan-Ping Wu

Richard Dana Johnson

Dijia Huang

Frank W Wogoman

Matthew K Musho

Christine D Nelson

Amy H Chu

Greg P Beer

Ronald G Sommer

Marvin A Genshaw

James B Denton

Brenda L Tudor

Lowry J Messenger

Brenda Tudor

H-Volker Runzheimer

A biosensor () for determining the presence or amount of a substance in a sample and methods of use of the biosensor () are provided. The biosensor () for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.

Biosensor for determining an analyte concentration

Method of using a biosensor

Oxidizable species as an internal reference for biosensors and method of use

Method and apparatus for correcting ambient temperature effect in biosensors

Disclosed is an improvement to the method of determining the concentration of a hemoglobin adduct in a blood sample by the steps of assaying the blood sample for the total amount of hemoglobin, assaying the blood sample for the hemoglobin adduct, and dividing the hemoglobin adduct concentration by the total hemoglobin concentration. The improvement involves normalizing the measurement of the hemoglobin adduct to the total amount of hemoglobin in the blood sample.

Method for the determination of hemoglobin adducts

A method for the determination of protein and creatinine in urine. An organic acid, in the form of its lithium salt, is dried onto a suitable substrate in the second reaction zone of a reaction vessel having a first reaction zone for the immunometric determination of the protein and a second reaction zone for the colorimetric determination of creatinine. After completion of the immunometric determination of the protein the dried organic salt is contacted with the urine containing creatinine resulting in solubilization of the organic acid salt as creatinine reactive reagent thereby facilitating the colorimetric determination of the creatinine.

Dry reagent for creatinine assay

Analytical reaction cassette and method for performing sequential analytical assay procedures to determine the amount of an analyte in a liquid test mixture. The reaction cassette can be in the form of a substantially square container having a substantially horizontal axis of rotation and incorporated with one or more analytical reagents such that they are contacted with a liquid test mixture in a desired ordered sequence to perform a particular assay procedure. Corners provided by the substantially square configuration of the reaction cassette disrupt the flow of liquids disposed in the reaction cassette upon contact therewith to thereby agitate and mix such liquids. A liquid disposed in the reaction cassette is capable of being manipulated and mixed therein by rotating the reaction cassette about the horizontal axis at sufficiently low velocities wherein the transport of such liquid is noncentrifugal and due substantially only to gravitational force. The reaction cassette is particularly useful for performing immunoturbidimetric assays, e.g., for the determination of glycated hemoglobin.

Reaction cassette for preforming sequential analytical assays by

Compositions and test articles comprisng ionophore of an optically active isomer of bis(12-crown-4-methyl)dialkyl malonate, wherein alkyl group is methyl and the second alkyl group has 4 to 20 carbon atoms, with a reporter substance incorporated in a hydrophobic carrier are disclosed.

Ionosphere and reporter substance incorporated into a hydrophobic carrier

The synthesis and use of optically active isomers of bis(12-crown-4-methyl) dialkylmalonates as sodium specific ionophores is described. The synthesis of optically active isomers of hydroxymethyl 12-crown-4 useful in synthesizing ionophores is also described.

Chiral crown ethers

A water-insoluble, crosslinked polyacrylamide copolymer is prepared having recurring monomeric residues of acrylamide, bisacrylamide or analogs thereof, and N,N'-bisacrylylcystamine or analogs thereof. The crosslinked copolymer can be formed into discrete particles having crosslinking disulfide groups on their external surface which can be reduced to provide activated particles having exposed chemically active sulfhydryl groups. The activated polyacrylamide particles are free of residual amino and carboxylic acid groups and can be reacted with suitable haptens and other biologically active substances to provide insolubilized forms thereof.

Crosslinked polyacrylamide-sulfhydryl polymer for immobilization of

Growing community of inventors

Elkhart, IN, United States of America

Kin-Fai Yip