Tohoku University

Tokyo University of Agriculture and Technology

Meiji Seika Pharma Co., Ltd.

Meui Seika Pharma Co., Ltd.

National University Corporation Tokyo University of Agriculture and Technology

Kimiko Watanabe

Masafumi Goto

Youhei Yamagata

Kazutaka Murayama

Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived fromsp. According to this method, the type and amount of protein-degrading enzyme to be used for isolating cells can be determined from the composition of a major protein of the biological tissue. Thus, cells having a high biological activity can be efficiently separated while reducing the amount of protein-degrading enzyme to be used.

Method for separating cell from biological tissue

The present invention relates to a method for obtaining cells or cell populations having a high biological activity from a biological tissue by enzymatic isolation, and probes for use in the method; more specifically to a method for analyzing a biological tissue, comprising applying two or more probes respectively containing biological-component binding domains through which two or more proteins bind to a predetermined biological component, to an isolated biological tissue, and analyzing binding amounts of the probes to the biological tissue.

Probe for analyzing biological tissue and method for utilizing same

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Sendai, Japan

Kimiko Watanabe