Syngenta Participations Ag

Kimberly Ann White

Paul Bernasconi

Thomas D Gaffney

Jennifer Audrey Hunt

Wenling Wang

Albert Flavier

Witold Filipowicz

Yanshan Ji

Robert Michael Petrovich

Gary Keith Powell

Ray Donald Carpenter

Jurgen Wendland

Frederick Dietrich

Peter Phillippsen

Michelle M Cloyd Kirksey

Er-Te Jamie Huang

A parallel high throughput method and kit for the discovery of small molecule interactors of target proteins, in particular, target proteins expressed from entire genomes or subsets thereof. The target module is prepared by binding selectively a target protein, modified to allow its detection, to a surrogate ligand that is linked to an individually detectable bead. Sets of target modules are mixed together in each chamber of a multi-chamber container. A test compound or collection of test compounds is added to each chamber, and the interaction of a compound with each target module is observed. A compound that is specific for a particular target protein will displace that protein from its target module. The identity of the target module so disrupted is determined by identifying the particular bead to which the displaced target protein was attached.

Parallel high throughput method and kit

The invention relates to genes isolated from,that code for proteins essential for normal fungal growth and development. The invention also includes the methods of using these proteins to discover new fungicides, based on the essentiality of the gene for normal growth and development. The invention can also be used in a screening assay to identify inhibitors that are potential fungicides.

Fungal target genes and methods

Growing community of inventors

Durham, NC, United States of America

Kimberly Ann White