Novozymes A/s

Novo Nordisk A/s

Novo Nordisk-a/s Novo Alle

Kim Uhre Hansen

Markus Sakari Kauppinen

Shamkant Anant Patkar

Torben Halkier

Claus Dambmann

Tina Spendler

Joan Qi Si

Peter Rahbek &Oslash;stergaard

Peter Rahbek Stergaard

Sune Jakobsen

Peter Rahbek Oestergaard

Svend Gunnar Kaasgaard

Poul Bach

Mads Aage Laustsen

Jørgen Topp Jørgensen

Carsten Jacobsen

Anders Jørgensen

Dan Oftelund

Gustav Borup Søndergaard

S&oslash;ren Bo Nielsen

A method of solubilizing protease crystals and/or protease precipitate in a fermentation broth comprising

Protease crystals in broth

The present disclosure relates to a method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth including: a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; and c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50&#xb0; C. to 80&#xb0; C. In accordance with the present disclosure, the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 60% of the initial value.

Nuclease reduction

The invention relates to a particle comprising an enzyme and a biomass, to a process for preparing a particle comprising spray drying an enzyme and biomass containing fermentation broth starting material, to obtain a solid particle comprising an enzyme and a biomass and to a process for preparing an enzyme containing particle comprising spray drying an aqueous enzyme containing liquid starting material to obtain a spray dried first enzyme containing particle and subsequently subjecting the first dry particle to a process selected from granulation and coating and combinations thereof to obtain a second dry enzyme containing particle.

Spray dried enzyme product

A microfiltration process of a fermentation-derived product comprising adding activated carbon to a solution of the fermentation-derived product prior to or during the microfiltration process at a microfiltration process temperature of from 25&deg; C. to 65&deg; C.

Microfiltration using activated carbon

The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

Enzymes with aminopeptidase activity

The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.

The present invention relates to a enzyme exhibiting aminopeptidase activity, a method for producting said enzyme, and enzyme preparation containing said enzyme exhibithing aminopeptidase activity, and use of said enzyme for various industrial purposes.

Growing community of inventors

Kalundborg, Denmark

Kim Uhre Hansen