Cellomics, Inc.

Life Technologies Corporation

Other

Kim Ippolito

Dirk John VandenBerg, Iii

Richard Salisbury

Laurel Rebecca Stone

Paul J Sammak

Ravi Kapur

Gustavo Rosania

Lawrence J Zana

Jason Bush

Alex Friedman

Michael Andrew Sipe

George Hanson

Audra Ziegenfuss

Erik Persmark

Jonathan Paullin

Zenia Wei

Oleg Lapets

Sarah Burroughs Tencza

Randall Shopoff

Ethan Melious

Ronald Albert Kuhn

Provided are systems and methods that allow a user to capture images at low- and high-level magnification and then overlay the high-level magnification images on the low-level magnification image to ease review of the images. The high-level magnification images may be overlaid on the low-level magnification image based at least in part on the portion of the low-level magnification image from which the high-level image was originated.

Systems, methods, and apparatuses for image capture and display

Microscope system display screen with graphical user interface

Accurate, reliable, and robust laser-based autofocus solutions are presented for through-the-lens microscope applications using slides or micro-titer plates. The laser-based autofocus solutions solve many of the problems that have arisen due to multiple reflective surfaces at varying distances relative to a sample of interest. The laser-based autofocus solutions provide a unique solution to resolve the ambiguity caused by these multiple reflective surfaces by using an image-based approach.

Image-based laser autofocus system

A stage assembly, an imaging system that uses the stage assembly, and methods for using the stage assembly in a high content screening system. The stage assembly includes a stage having a top surface and an opposing bottom surface and an opening extending between the top and bottom surfaces to receive a specimen plate. The stage assembly also includes a calibration sample bay formed in the stage. A calibration sample can also be secured within the calibration sample bay.

Integrated calibration sample bay for fluorescence readers

The present invention provides a test plate and methods for adjusting fluorescence imaging systems involving using a plate with fluorescent microbeads bound to a surface.

Microbead-based test methods for fluorescence imaging systems

The invention provides a method for a method for determining a best initial focal position estimate for a current sample location on a substrate comprising multiple sample locations, comprising determining the best initial focal position estimate by using a result from one or more techniques selected from the group consisting of linear regression analysis of focal positions determined for at least two other sample locations on the substrate and quadratic regression analysis of focal positions determined for at least three other sample locations on the substrate.

Method of determining a best initial focal position estimate

Microbead-based test plates and test methods for fluorescence imaging systems

Growing community of inventors

South Park, PA, United States of America

Kim Ippolito