Organo Corporation

Japan Organo Co., Ltd.

Kikuzo Kaneko

Fumihiko Matsuda

Takayuki Masuda

Kohei Sato

Kouji Tanikawa

Tohru Sonobe

Isamu Miyawaki

Makoto Tomizawa

In chromatographic separation in a plurality of steps with a chromatographic separator packed with an ion exchanger used as at least part of chromatographic packing, at least part of the ionic form of at least part of the ion exchanger is changed to an ionic form fit for separation of components to be separated in each step to effect chromatographic separation, whereby a starting fluid material containing at least 3 components can be efficiently separated into at least 3 fractions. A preferred example of the chromatographic separator comprising a group of packing bed units linked in endless series to form a circulation flow path is provided with a shutoff valve at a position of the circulation flow path; starting fluid material feed means, desorbent feed means and 2-fraction withdrawal means for all the packing bed units; and a single-fraction withdrawal means connected to the circulation flow path on the upstream side of the shutoff valve and without any packing bed unit therebetween.

Chromatographic separation process and chromatographic separator

At least 2 different packings differing in the resolution of components contained in a starting fluid material such as a starting solution material and desired to be separated are used in a coexistent state to adjust the resolution of the components. The starting fluid material is flowed through the resultant packing bed to effect chromatographic separation, whereby high-purity fractions enriched with respective components can be obtained at high recoveries with a small amount of desorbent such as eluent. In a chromatographic separator, at least 2 packings comprising a first packing high in the resolution of a component having a medium affinity (affinity for packing) and a weak-affinity component and a second packing high in the resolution of a strong-affinity component and the medium-affinity component in a coexistent state are used as chromatographic packing in contact with a fluid containing the medium-affinity component to adjust the resolution of every two of at least 3 components, whereby at least 3 fractions can be obtained.

Method and equipment for chromatographic separation

Chromatographic separation process

In demineralization of a beet sugar solution before boiling as the starting solution with a simulated moving bed chromatographic separator comprising a plurality of packed column units packed with a strongly acidic cation exchange resin in a salt form having an average grain size of 300 to 500 &mgr;m and a uniformity coefficient of at most 1.2 as chromatographic packing (separating packing), and each provided with a starting solution feed inlet, an eluent water feed inlet, a withdrawal outlet for a fraction of a component having a strong affinity for chromatographic packing (&ldquo;strong-affinity component&rdquo;), and a withdrawal outlet for a fraction of a component having a weak affinity for chromatographic packing (&ldquo;weak-affinity component&rdquo;); use is made of circulating pumps, the number of which is at least half of the number of the packed column units, and the beet sugar solution before boiling is demineralized under the following conditions: starting solution feed rate/eluent water feed rate volume ratio&equals;1/2.5 to 1/3.5, strong-affinity component fraction withdrawal rate/weak-affinity component fraction withdrawal rate volume ratio&equals;0.5/1 to 1.2/1, and circulating flow rate&equals;25 to 80 vol. % /hr. based on chromatographic packing in a slowest flow velocity zone in the circulation system, whereby a sucrose fraction having a high sucrose concentration is obtained using a small amount of eluent water with a large throughput.

Process for demineralizing a sugar solution

Process for recovering betaine

Disclosed is a process for producing high-purity glucose by saccharifying liquefied starch with the aid of an enzyme, in which the saccharification reaction of the liquefied starch is discontinued at a point where the glucose content is less than 96% by weight, preferably within the range of 80 to 93% by weight, on a solid basis. The resulting saccharified solution is filtered, concentrated and softened as required, and then fractionated by subjecting it to column chromatography. Thereafter, the resulting glucose fraction is recovered. This process makes it possible to produce high-purity glucose efficiently in spite of a reduction in saccharification time.

Process for the production of glucose

There is provided a process for efficient chromatographic separation of a plurality of components from a fluid feed containing the same, which process comprises repeating a cycle comprising the step (1) of substantially shutting off the internal fluid circulation at a position of endless, or continuous, circulatory system and withdrawing a fraction enriched with a component having a strong affinity for an adsorbent upstream of the shut-off position while feeding the fluid feed downstream of the shut-off position, and the step (2) of withdrawing a plurality of fractions enriched with the remaining components while feeding a fluid desorbent and circulating the internal fluid.

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Tokyo, Japan

Kikuzo Kaneko