Stokes Bio Limited

Gencell Biosystems Limited

Anucell Biosystems Limited

Becton Dickinson and Company Limited

Kieran Curran

Mark Davies

Tara Dalton

David McGuire

Brian T Chawke

Noel Sirr

Paul Fleming

Damian Michael Curtin

Brian Barrett

Micheál Keane

Patrick Tuohy

Julie Garvey

Inga Rosca

Caitriona Ryan

Mark McCabe

Macdara Glynn

Martin Reddin

John P Griffin

Jason Ryan

David Merrigan

John Daly

Seamus Gilhooley

Michael Sayers

Séamus Gillhooley

John Gerard Daly

Conor McCarthy

Ryan J Talbot

Mauro Aguanno

David Kinahan

Damien King

Robert Roeven

Mark Korenke

Damian Curtain

Xiaona Hou

Micheal Keane

Mark Mccabe

John Paul Griffin

David Mcguire

A system for processing a biological sample can include a droplet generation assembly comprising a plurality of first reservoirs configured to contain an aqueous sample and a plurality of second reservoirs configured to contain a carrier fluid immiscible with the aqueous sample. The plurality of first reservoirs and the plurality of second reservoirs can be arranged to be in respective flow communication in pairs of reservoirs comprising a first reservoir of the plurality of first reservoirs and a second reservoir of the plurality of second reservoirs constituting a plurality of pairs of reservoirs. The droplet generation assembly can further include a flow control system configured to control a pressure in the plurality of pairs of reservoirs so as to generate a flow of a series of volumes of the aqueous sample separated by the carrier fluid. The system can further include a thermocycling system.

Liquid bridge and system

A system for processing a biological sample includes a substrate comprising a plurality of wells and a plurality of flow channels. The system further includes a flow control system comprising a manifold having a plurality of ports configured to fluidically couple to the plurality of wells, and one or more containment structures configured to contain carrier fluid and fluidically couple to the ports. The flow control system further includes a cradle configured to removably receive the substrate. The flow control system is configured to transmit pressure differential, via the manifold, to the plurality of wells so as to cause a plurality of sample volumes held by at least some wells of the plurality of wells to flow through respective flow channels and cause the carrier fluid to flow through the flow channels and form a plurality of droplets of the plurality of sample volumes separated by the carrier fluid.

System for processing biological sample

Assembly of bioreactor components

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower portion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

Torus reactor for a combined cell isolator and bioreactor

Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower potion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

A bridge () comprises a first inlet port () at the end of a capillary, a narrower second inlet port () which is an end of a capillary, an outlet port () which is an end of a capillary, and a chamber () for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port () causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (), and the droplets combine at the liquid bridge (). In another embodiment, a droplet () segments into smaller droplets which bridge the gap between the inlet and outlet ports.

Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.

Biological detection system and method of use

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple nodes; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each node of the thermal chip module.

Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same

Devices, systems and methods for making and handling liquid samples are disclosed.

Handling liquid samples

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple CLC reaction wells; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each CLC reaction well of the thermal chip module.

Benchtop nucleic acid library preparation device and methods for using the same

A sample handling method may include drawing an encapsulating liquid from an encapsulating-liquid input; discharging the drawn encapsulating liquid (a) onto a free surface of a carrier liquid in a carrier-liquid conduit comprising a stabilisation feature and (b) proximate to the stabilisation feature, the encapsulating liquid being immiscible with the carrier liquid, so that the discharged encapsulating liquid does not mix with the carrier liquid, floats on top of the carrier liquid, and is immobilised by the stabilisation feature; drawing a sample liquid from a sample-liquid input; and discharging the drawn sample liquid, the sample liquid being immiscible with the encapsulating liquid and with the carrier liquid, so that the sample liquid does not mix with the encapsulating liquid or with the carrier liquid.

Composite liquid cells

Methods, devices and systems for handling sample liquids, encapsulating liquids and magnetic particles are disclosed.

Biomolecule isolation

Biomolecule isolation and thermal processing

Devices, systems, and methods for charging fluids are disclosed. The charging of fluids improves the mixing of fluids in microfluidic systems. The charging is performed by producing an ion field between an ionizing electrode and an opposed ground electrode. A fluid-containing vessel is positioned between the opposed electrodes and the ion field charges the fluid in the vessel.

System and method for charging fluids

Growing community of inventors

Ballyclough, Ireland

Kieran Curran