Korea University Research and Business Foundation

The General Hospital Corporation

Ki Wan Bong

Hyeon Ung Kim

Hyun Yeul Lee

Daniel Irimia

Yoon Ho Roh

Hyun June Moon

Seok Joon Mun

The present invention relates to a method for fabricating microparticles by using a degassed gas-permeable micro-mold and discontinuous dewetting. The method for fabricating microparticles according to the present invention comprises the steps of: depressurizing and degassing a porous micro-mold including a plurality of micro-wells concavely recessed in a predetermined shape and size from one surface thereof (S); loading a microparticle precursor solution on which the micro-wells are formed, and covering the microparticle precursor solution with a cover substrate (S); moving the cover substrate to the side (S); and curing the microparticle precursor solution filled in the micro-wells, thereby synthesizing microparticles (S).

Method for fabricating microparticle by using degassed gas-permeable micromold and discontinuous dewetting

The present invention provides a method for producing microparticles in which a porous micromold is degassed such that a precursor solution is quickly loaded into the micromold through the suction force of the micromold regardless of the viscosity and wettability of the precursor solution.

Method for producing microparticles

The present invention relates to a method for preparing encoded hydrogel particles for high sensitive detection of a target biomolecule with high accuracy, and encoded hydrogel particles prepared thereby and, specifically, to a method for preparing encoded hydrogel particles, comprising a step of synthesizing hydrogel particles, and then binding a probe thereto, and encoded hydrogel particles prepared thereby. According to the present invention, probes can be loaded with remarkably improved high efficiency, loaded probes can be uniformly distributed, and the potential problem of biomolecule detection inhibition caused by an unreacted end can be resolved. In addition, the present invention can be applied to the diagnosis of diseases or screening of drugs through high sensitive multiplex detection of target biomolecules such as nucleic acids and proteins, and therefore, can be widely used in the field of medical diagnosis including molecular diagnosis.

Method for preparing encoded hydrogel particles, and encoded hydrogel particles prepared thereby

Methods for sorting cells include: arranging microparticles into an array on a substrate in a microfluidic device, in which the microparticles each include multiple reference markers; introducing multiple cells to the array of microparticles under conditions that enable at least some of the cells to adhere to the microparticles; removing the microparticles, to which the cells are adhered, from the substrate; transferring the microparticles, to which the cells are adhered, to a detection region; and detecting, for each of two or more microparticles that pass through the detection region, a microparticle feature; and sorting the two or more microparticles based on the detected features, in which the detected features are related to a phenotype of the cells.

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Seoul, South Korea

Ki Wan Bong