Northwestern University

Ki-Hee Song

Hao F Zhang

Cheng Sun

A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order ('1') light and a negative first order ('&#x2212;1') light using a grating; wherein the 1light comprises spectral information and the &#x2212;1light comprises spectral information; capturing the 1light and the &#x2212;1light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (&#x201c;DWP&#x201d;) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

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Evanston, IL, United States of America

Ki-Hee Song