Hanmi Pharm. Co., Ltd.

Hanmi Science Co., Ltd.

Hanmi Holdings Co., Ltd.

Forhumantech Co., Ltd.

Ki-Doo Choi

Se Chang Kwon

Young Jin Park

Sung Youb Jung

Gwan Sun Lee

Gwan-Sun Lee

Jin Sun Kim

Se-Chang Kwon

Sung-Youb Jung

Sang-Kyou Lee

Seung-Kyou Lee

Sung-Min Bae

Cha-Soon Kim

Hoon Shin

Jay-Do Choi

Disclosed are a recombinant expression vector comprising a nucleotide sequence encoding an-derived signal sequence and a nucleotide sequence encoding an immunoglobulin constant region, and a transformant transformed with the expression vector. Also, disclosed is a method of mass-producing an immunoglobulin constant region by culturing the transformant and expressing the immunoglobulin constant region in a water-soluble form.

Method for the mass production of immunoglobulin constant region

The present invention relates to methods of delivering nucleic acids into cells using a nucleic acid binding molecule containing a multimeric or spacer-incorporated protein transduction domain (PTD). The invention also relates to novel compositions that contain a nucleic acid complexed or conjugated with a nucleic acid binding molecule. The nucleic acid binding molecule may contain a multimeric or spacer-incorporated PTD, and may further contain a nucleic acid binding region. The nucleic acid complexes or conjugations of the present invention may be employed to inhibit expression of a target gene, and/or determine the function of a target gene.

Pharmaceutical compositions and methods for delivering nucleic acids into cells

Disclosed in this invention are an expression vector for the secretive production of human interferon alpha (hIFN&#x3b1;) comprising a polynucleotide encoding a modifiedthermostable enterotoxin II signal sequence and a polynucleotide encoding hIFN&#x3b1; ligated to the 3'-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, the process being capable of secreting a soluble form of active hIFN&#x3b1;, which does not contain an additional methionine residue at its N-terminal, into the periplasm of ancell.

Expression and secretion vector for human interferon alpha and process for producing human interferon alpha by employing same

A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, the modified enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th, and 22nd amino acids of enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm.

Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of a said peptide and a heterologous protein

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Seoul, South Korea

Ki-Doo Choi