Health Research, Inc.

The State University of New York

The Brigham and Women's Hospital, Inc.

Khushi L Matta

Rakesh K Jain

Robert Sackstein

Sriram Neelamegham

Ralph J Bernacki

Charles J Dimitroff

Robert D Locke

E V Chandrasekaran

Edaya V Chandrasekaran

Moheswar Sharma

Jun Xue

Brajeswar Paul

A method for producing sialylated molecules based on reverse sialylation that catalytically transfers the sialic acid moiety of sialylated donors to nucleotide monophosphates or transfer sialic acid moieties from sialylated donors to acceptor glycoproteins or glycolipids.

Method for synthesis of sialylated products using reversible sialylation

The present invention provides compositions and methods for inhibiting cell migration, e.g., lymphocytes and inflammation. The invention also provides an improved process for preparing fluorinated N-acetylglucosamines.

Fluorinated glucosamine analogs useful for modulating post-translational glycosylations on cells

Compounds which bind to selectin receptors and thus may modulate the course of inflammation, cancer and related processes by intervening with cell-cell adhesion events. Further, such compounds can be used for identification and analysis of such receptors. In this regard the invention is directed to compounds of formula (I). ##STR1## wherein R.sup.1 is independently H, alkyl, aryl, an aryl alkyl, alkenyl or one or more additional saccharide residues; R.sup.2 =H or OH provided that when R.sup.2 is H, R.sup.3 is OH; R.sup.3 =H or OH provided that when R.sup.3 is H, R.sup.2 is OH; X=H, SO.sub.3.sup.- or PO.sub.4.sup.- ; Y is independently H, OH, OR.sup.4 or NHCOR.sup.4, wherein R.sup.4 is alkyl, and Z is an organic acid residue. .alpha.-L-Fucose residue can be modified or replaced with suitable bioisosters or a different saccharide residue such as D-mannose. Modification of L-fucose may include replacement of each or all of the hydroxyl groups with H or OR' wherein R' can be methyl, ethyl or allyl groups.

Synthetic core 2-like branched structures containing GalNAc-lewis.sup.x

A biologically active oligosaccharide compound comprising at least two L hexose rings connected together by an ether oxygen atom. The ether oxygen atom is connected to a first of the rings at the first carbon atom to the right of the hexose ring oxygen atom. The compound contains at least one sulfate or phosphate group connected to the first ring at the third carbon atom to the right of the ring oxygen or to a methyl group at the fifth carbon atom to the right of the ring oxygen atom. The invention further includes the method for using the above oligosaccharide compound to detect .alpha. 1,3-L-fucosyltransferases or to block the activity of such .alpha. 1,3-L-fucosyltransferases or structures which mimic the structure of such fucosyltransferases to the extent that such structures bind to compounds of the present invention, e.g., as in the case of HIV virus.

Acceptor for fucosyl transferase

A novel, efficient glycosylating reagent, R 3,4-O-isopropylidene-2-O-(4-methoxybenzyl)-1-thio-.beta. -L-fucopyranoside wherein R is a lower alkyl or lower alkenyl of 1 to 8 carbons such as methyl or pentenyl, is described. This novel reagent provides for the facile synthesis of linear and other .alpha.-L-fucosyl oligosaccharides. In addition, a method for the preparation of 2'-O-.alpha.-L-fucopyranosyl-lactose, which comprises reacting the abovementioned glycosylating reagent with solvated 4-O-(6-O-acetyl-3,4-O-isopropylidene-.beta.-D-galactopyranosyl)-2,3,5,6-di -O-isopropylidene-D-glucose, dimethyl acetal, a tetrabutylammonium halide and a catalyst and then de-O-acetylating the resulting reaction product with sodium methoxide methanol, is also disclosed.

Glycosylating reagent for the synthesis of linear and other

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Williamsville, NY, United States of America

Khushi L Matta