Southern Illinois University

University of Missouri

Khalid Meksem

David A Lightfoot

Melissa Goellner Mitchum

Pramod Kaitheri Kandoth

Shiming Liu

Naoufal Lakhssassi

Dounya Knizia

Paul T Gibson

Zhou Zhou

The present invention is directed to a transgenic soybean plant with increased oleic acid content comprising a polynucleotide encoding a fatty acid desaturase (FAD) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAD activity. The invention is also related to a plant of an agronomically elite soybean variety with increased oleic acid content comprising a polynucleotide encoding a fatty acid desaturase (FAD) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAD activity. The invention is still further directed to a method of increasing the oleic acid content of a soybean plant comprising transforming the soybean plant with a polynucleotide encoding a fatty acid desaturase (FAD) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAD activity.

Compositions and methods to increase oleic acid content in soybeans

The present invention is directed to a transgenic soybean plant having increased oleic acid content comprising a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity. The invention is further directed to a method of increasing oleic acid content of a soybean plant comprising transforming a soybean plant with a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity.

Soybean lines with low saturated fatty acid and high oleic acid contents

A transgenic soybean plant or parts thereof, resistant to soybean cyst nematodes, transformed to express Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof. Also provided is a method of making such a plant. Also provided is an artificial DNA construct encoding Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof.

Soybean resistant to cyst nematodes

A transgenic soybean resistant to soybean cyst nematode (SCN), or parts thereof, including an artificial DNA construct encoding a serine hydroxymethyltransferase protein (e.g., GmSHMT). Also provided are GmSHMT alleles containing mutations R130P and Y358N along with research and breeding methods and compositions including such.

Soybean cyst nematode and soybean sudden death syndrome resistance genes, soybean cyst nematode and soybean sudden death syndrome resistant plant lines, and methods of breeding and engineering same.

Isolated soybean cyst nematode and soybean sudden death syndrome polypeptides

A method of determining the presence of soybean sudden death syndrome resistance in the soybean plant in a greenhouse setting, the method comprising the steps of: (a) inoculating soil with a low density inoculum of; (b) planting a soybean plant in said inoculated soil; (c) growing said plant in said soil in a greenhouse; (d) isolating-infected tissue from said plant; (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth; (f) scoring at least one of disease severity and infection severity in said plant using the number of said fungal colony forming units; and (g) correlating at least one of said disease severity and said infection severity to at least one of disease severity and infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, wherein a statistically significant correlation indicates presence of soybean sudden death syndrome resistance in said soybean plant. Also provided is a method of characterizing resistance to soybean sudden death syndrome in a soybean plant, the method comprising the steps of: (a) isolating roots from a soybean plant infected by; (b) culturing the root on a culture plate including a restrictive growth medium that provides for slow fungal growth and restricted bacterial growth; (c) determining root infection severity by statistically evaluating the number ofcolony forming units on said culture plate; and (d) characterizing resistance to soybean sudden death syndrome in said soybean plant based on said determined root infection severity.

Growing community of inventors

Carbondale, IL, United States of America

Khalid Meksem