Applied Biosystems, LLC

Applera Corporation

Life Technologies Corporation

The Perkin-elmer Corporation

Pe Corporation (ny)

Other

Pe Corporation

Khairuzzaman Bashar Mullah

Zhaochun Ma

Linda G Lee

Ronald J Graham

Timothy M Woudenberg

Kenneth J Livak

Muhammad A Sharaf

Francis T Haxo

Barnett B Rosenblum

Robert G Eason

Wanli Bi

Scott C Benson

Jeffrey M Marmaro

Steven Menchen

Jacob K Freudenthal

Susan J Flood

Ravi S Vinayak

Jeffrey Mamoro

Brian Lee Evans

Xiongwei Yan

Susan J A Flood

Joe Y Lam

William A Andrus

Gregory J Ewing

Chu-An Chang

The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure.

Modified rhodamine dye and use thereof in biological assays

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Quencher and reporter dye combinations

The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.

Methods of detecting target nucleic acids

Methods for preparation of 2',3&#x2032;-dideoxynucleotides support structures, such as 2&#x2032;,3&#x2032;-dideoxyguanosine, 2&#x2032;,3&#x2032;-dideoxyadenosine, and 3&#x2032;-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

Synthesis of 2',3&#x2032;-dideoxynucleosides for automated DNA  synthesis and pyrophosphorolysis activated polymerization

Compositions, methods and kits for labeling proteins and uses in reporter systems for detecting, quantifying and/or characterizing analytes.

Use of antibody-surrogate antigen systems for detection of analytes

Synthesis of 2', 3&#x2032;-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

Synthesis of 2',3&#x2032;-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.

Non-fluorescent quencher compounds and biomolecular assays

Disclosed, among other things, are primers containing certain modified nucleobases in the 3' terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.

Modified oligonucleotides  and applications thereof

Modified oligonucleotides and applications thereof

Growing community of inventors

Union City, CA, United States of America

Khairuzzaman Bashar Mullah