Pacific Biosciences of California, Inc.

Roche Sequencing Solutions, Inc.

Applied Biosystems, LLC

Khai Luong

Brian Christopher Godwin

Igor D Vilfan

Jan Berka

Sedide Ozturk

Joseph Platzer

Yu-Chih Tsai

Carolina Dallett

Seoyoung Kim

Florian Rubelt

Richard Dannebaum

Jainee Lewis

Stephen Wilson Turner

Steven Menchen

Paul M Lundquist

John Lyle

Handong Li

Meng Taing

Christina E Inman

Ravi Dalal

Dilduz Telman

Darya Filippova

Garima Kushwaha

Igor Drasko Vilfan

Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

Immune profiling by primer extension target enrichment

Described herein are methods for identifying quasispecies of genomes by clustering sequence reads for samples including the genomes based on the similarities of the sequence reads.

Direct variant phasing in long reads to detect quasispecies

Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of the initial population enriched for a target region, which is typically located within one or more target fragments. The methods are particularly useful for analyzing populations having a high degree of complexity, e.g., chromosomal-derived DNA, whole genomic DNA, or mRNA populations.

Compositions and methods for enrichment of nucleic acids

Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.

Methods and systems for analysis of fluorescent reactions with modulated excitation

Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol), where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and 'Pol' represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby

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Oakland, CA, United States of America

Khai Luong