Grifols Worldwide Operations Limited

Grifols, S.a.

Kevin Wee

Thomas P Zimmerman

James Rebbeor

Charles Miller

Eric Allgaier

Anthony Klos

Michelle StPeter

Kelly Glancy

Zihao Wang

Jessica Sloan

Compositions include highly concentrated Alpha-1 Proteinase Inhibitor (A1PI) in a concentration greater than or equal to 100 mg/ml. Pharmaceutical compositions can be prepared from these compositions. The pharmaceutical compositions can be suitable for subcutaneous administration. The highly concentrated A1PI solutions can be obtained by single-pass tangential flow filtration (SPTFF).

Composition comprising highly-concentrated Alpha1 proteinase inhibitor and method for obtaining thereof

Compositions include highly-concentrated Alpha-1 Proteinase Inhibitor (A1PI) in a concentration greater than or equal to 100 mg/ml. Pharmaceutical compositions can be prepared from these compositions. The pharmaceutical compositions can be suitable for subcutaneous administration. The highly-concentrated A1PI solutions can be obtained by single-pass tangential flow filtration (SPTFF).

Composition comprising highly-concentrated &#x3b1;1 proteinase inhibitor and method for obtaining thereof

Composition comprising highly-concentrated ALPHA1 proteinase inhibitor and method for obtaining thereof

The invention relates to methods and kits for the analysis of the sialylation of gluco-proteins. The samples of gluco-protein are incubated separately under three conditions: with beta-galactosidase, with beta-galactosidase+alpha-sialidase, and without an enzyme. After the enzyme treatment, high performance anion exchange chromatography with pulsed amperometric detection (HPAEC PAD) is used to make a quantitative determination of the total galactose in the sample, the non sialylated galactose and the exogenous galactose in the medium. The determination of said values makes it possible to deduce the percentage of sialylation of the gluco-protein.

Rapid and accurate analysis of protein sialylation

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Wake Forest, NC, United States of America

Kevin Wee