Synthetic Genomics, Inc.

Metabasis Therapeutics, Inc.

Kevin V Martin

Stephen K Picataggio

Kevin T Watts

Robert Christopher Brown

Spiros Kambourakis

Gena Roy

Jessica Roxane Kristof

Benjamin M Griffin

Prachee Prakash

Stuart A Underwood

Paul Gordon Roessler

John Verruto

Markus Herrgard

Kathleen Reeves

The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

Compositions and methods for producing chemicals and derivatives thereof

Method for synthesizing FDCA and derivates thereof

This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks.

Methylbutanol as an advanced biofuel

The invention provides a mechanism for semi-quantitatively measuring individual isotopomer species of a molecule using gas chromatograph mass spectrometry. The method allows for semi-quantitatively tracking the movement of ions by measuring the individual isotopomer species of a molecule.

Mass spectrometry method

This invention describes genes, metabolic pathways, microbial strains and methods to produce 2,6-dimethyloctane as an advanced biofuel from renewable feedstocks.

Dimethyloctane as an advanced biofuel

This invention describes genes, metabolic pathways, microbial strains and methods to produce methyl butanol and other compounds of interest from renewable feedstocks.

Methods for the synthesis of cyclic phosphonic acid diesters from 1,3-diols are described, whereby cyclic phosphonic acid diesters are produced by reacting a chiral 1,3-diol and an activated phosphonic acid in the presence of a Lewis acid.

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Solana Beach, CA, United States of America

Kevin V Martin