Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

University of California

Kevin T Chapman

Mark P White

J Tanner Nevill

Daniele Malleo

Randall D Lowe, Jr

Jason M McEwen

Gang F Wang

Andrew W McFarland

Steven W Short

Xiaohua Wang

M Jimena Loureiro

Xiao Guan

Eric D Hobbs

Aathavan Karunakaran

Kristin G Beaumont

Natalie C Marks

Guido K Stadler

Igor Y Khandros

Gaetan L Mathieu

Keith J Breinlinger

Ming C Wu

Alexander J Mastroianni

Jian Gong

Justin K Valley

Xiao Guan Radstrom

Minha Park

Gregory G Lavieu

Ming-Chiang Wu

Peggy A Radel

Yelena Bronevetsky

George L Fox

Peter J Beemiller

Shao Ning Pei

Annamaria Mocciaro

Keith Joseph Breinlinger

Kai W Szeto

Jodi Tsu-An Loo

Ravi K Ramenani

Kevin D Loutherback

Jason M Mcewen

Amanda L Goodsell

Christine E Sun

Duane Smith

Hariharasudhan Chirra Dinakar

Adrienne T Higa

Andrew W Mcfarland

Jr Randall D Lowe

Jason C Briggs

Jr Lowe Randall D

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

Freezing and archiving cells on a microfluidic device

Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

Micro-fluidic devices for assaying biological activity

A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor

Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (D) that separates activated T lymphocytes from na&#xef;ve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from na&#xef;ve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

Sorting of T lymphocytes in a microfluidic device

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device

The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

Tumor infiltrating cells engineered to express a pro-inflammatory polypeptide

Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.

Microfluidic reporter cell assay methods and kits thereof

Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

Pens for biological micro-objects

Growing community of inventors

Santa Monica, CA, United States of America

Kevin T Chapman