The General Hospital Corporation

The Charles Stark Draper Laboratory, Inc.

Massachusetts Institute of Technology

Kevin R King

Jeffrey T Borenstein

Joseph P Vacanti

Martin L Yarmush

Eli J Weinberg

Mohammad Reza Kaazempur-Mofrad

Chiao-Chun Wang

Alexander Sevy

Young-Moon Michael Shin

Jennifer Ogilvie

Tomoyuki Maemura

Osamu Ishii

Hidetomi Terai

Osamu Ishi

Suraj J Patel

John M Milwid

Eli The Charles Stark Draper Lab Inc Weinberg

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Fabrication of vascularized tissue using microfabricated two-dimensional molds

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Fabrication of tissue lamina using microfabricated two-dimensional molds

The invention provides for translating two-dimensional microfabrication technology into the third dimension. Two-dimensional templates are fabricated using high-resolution molding processes. These templates are then bonded to form three-dimensional scaffold structures with closed lumens. The scaffolds can serve as the template for cell adhesion and growth by cells that are added to the scaffolds through the vessels, holes or pores. These scaffolds can be formed by layering techniques, to interconnect flat template sheets to build up a fully vascularized organ.

Multilayer device for tissue engineering

Growing community of inventors

Western Springs, IL, United States of America

Kevin R King