Tokitae LLC

General Electric Company

Kevin Paul Flood Nichols

Bernhard Hans Weigl

Benjamin K Wilson

David Joseph Gasperino

Samantha A Byrnes

Toan Huynh

Ozgur Emek Yildirim

Matthew P Horning

David Michael Cate

Victor Petrovich Ostroverkhov

David Roger Moore

Matthew Jeremiah Misner

Ralf Lenigk

Mark Marshall Meyers

Phillip Rutschman

Bing Li

Joshua Davis Bishop

Benjamin David Grant

Damian Madan

Matthew F Rosen

Terry Lee Saunders

Timothy Toepfer

Keith Michael Looney

Caitlin E Anderson

Daniel Keith Connors

Phil Rutschman

David Gasperino

An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Determining a bulk concentration of a target in a sample using a digital assay with compartments having nonuniform volumes

Described herein are systems and methods for multiplexed analysis of two or more targets in a test sample including a first set of particles including a first set of target-specific reagents and a first optically detectable identifier capable of emitting a first wavelength indicative of a first target, and at least one second set of particles including a second set of target-specific reagents and a second optically detectable identifier capable of emitting a second wavelength indicative of a second target; and at least one optically detectable reporter probe capable of constitutively emitting a third wavelength in response to reaction of the first set of target-specific reagents with the first target in the test sample and/or reaction of the second set of target-specific reagents with the second target in the test sample, wherein the first wavelength, the second wavelength, and the third wavelength are optically discernable from one another.

Systems and methods for particle multiplexing in droplets

Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Assay structures for multi-step biochemical assays

A rapid diagnostic testing device for testing of a biological sample is provided. The device comprises a channeled construct, at least one lateral flow unit, and a cassette housing. The channeled construct is configured to receive a biological sample to form at least partially purified biological sample. The lateral flow unit is at least partially disposed in the cassette housing. The lateral flow unit comprises: a sample receiving zone, a conjugate zone and a detection zone. The sample receiving zone is operatively coupled to the channeled construct for receiving the partially purified biological sample comprising at least one analyte. The conjugate zone comprising a conjugate particle to bind the analyte is disposed adjacent to the first side of the sample receiving zone. The detection zone is disposed adjacent to the second side of the sample receiving zone and comprises at least one binding agent for detecting the analyte.

Device for rapid diagnostic tests to detect antigens with improved sensitivity

Embodiments disclosed herein are directed to flow assays including at least one electrically-actuated valve configured to control fluid flow. Methods of operating such flow assays are also disclosed.

Flow assay with at least one electrically-actuated fluid flow control valve and related methods

Disclosed is a low-cost, portable photo thermal spectroscopy (PTS) reader for use in detecting the presence of diseases in the bodily fluid of affected patients. The PTS reader is designed to be durable, easy to use and provide readings from the Lateral Flow Assay (LFA) with rapid results. Also provided are methods of use.

Fixed optics photo-thermal spectroscopy reader and method of use

Embodiments disclosed herein are directed to a photothermal spectroscopy assay reader including a light source configured to emit light in the red range of the light spectrum. Methods of operating such photothermal spectroscopy assay readers are also disclosed. Assay kits including a lateral flow assay and a photothermal spectroscopy assay reader configured to read the photothermal spectroscopy assay reader are also disclosed.

Photothermal spectroscopy assay readers, and related assay kits and methods

Embodiments disclosed herein are directed to photothermal spectroscopy apparatuses and systems for offset synchronous testing of flow assays. Methods of using and operating such photothermal spectroscopy systems are also disclosed.

Photothermal spectroscopy systems for offset synchronous testing of flow assays and methods of using same

Growing community of inventors

Issaquah, WA, United States of America

Kevin Paul Flood Nichols