Freie Universitat Berlin

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Oxford University Innovation Limited

Kevin Pagel

Peter H Seeberger

Rainer Haag

Carol Robinson

Heung Sik Hahm

Peter Seeberger

Hsin-Yung Yen

Svenja Christina Ehrmann

Leonhard Hagen Urner

Idlir Liko

Johanna Hofmann

Carol V Robinson

A method of detecting a protein by mass spectrometry comprises: providing a solution comprising a dendritic detergent and a protein; providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; vaporising the solution using the nanoelectrospray ionisation source; ionising the protein; resolving the ionised protein using the mass analyser; and detecting the resolved protein using the detector. A method of extracting a membrane protein from its native membrane comprises: providing a membrane protein in its native membrane; contacting the membrane protein with a dendritic detergent. Dendritic detergents for use in the methods are also provided.

Dendritic detergents for the analysis of proteins by mass spectrometry

The present invention relates to a method for determining in an expedient manner and with minimal sample consumption the structure of an unknown carbohydrate by using ion mobility-mass spectrometry (IM-MS) in negative ionization mode and fragmentation and a database containing structures of carbohydrates and/or of the fragments of the negative ions of carbohydrates, and for each of the structures of the target carbohydrates the collision cross section value and the mass-to-charge ratio value of the negative ion thereof, and for each of the structures of the fragments of the negative ions of the target carbohydrates the collision cross section value and the mass-to-charge ratio value of the fragment of the negative ion of the target carbohydrate.

Method for determining carbohydrates structure

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Berlin, Germany

Kevin Pagel