Bio-rad Laboratories, Inc.

Kevin P Raley

Daniel Nelson Fox

Susan Hunter

Nathan Michael Gaskill-Fox

Matthias J G Ottenberg

Richard A Miles

Tidhar Sadeh

Matthias J Ottenberg

Matthias JG Ottenberg

Disclosed is an automated method and apparatus for automatically setting a drop delay period by detecting calibration particles in a waste stream. The drop delay is incremented over a series of drop delays and the number of calibration particles in the waste stream is detected for each drop delay. The drop delay is selected which has the least number of calibration particles in the waste stream.

Automated and accurate drop delay for flow cytometry

Disclosed is a nozzle assembly that is compact in size and that uses plastic tubing as an injection needle. Standard plastic fittings are utilized, which are inexpensive and widely available. The nozzle assembly has a simple construction and can be easily assembled and disassembled in a few minutes by a user. Cleaning and/or replacement of parts is inexpensive. Plastic tubing can be used as an injection needle that has superior qualities over commonly used stainless steel injection needles. Flexure of the injection needle tubing is prevented because of the compact size of the nozzle cavity.

Condensed geometry nozzle for flow cytometry

Disclosed is an optical combiner for combining multiple laser beams in a flow cytometer. A dichroic beam combiner is used to combine a second laser beam with a first laser beam so that the two beams are collinear. A beam size adjuster is utilized to adjust the size and convergence/divergence of the second laser beam so that both laser beams focus in a vertical direction at the same location on a stream in the flow cytometer. A cylindrical lens with a vertically oriented axis in the focusable beam shaping optics can also be adjusted to adjust the location of the focus point of the two beams in the horizontal direction. Alignment is maintained with the opto-mechanical adjustments made on one laser beam relative to the other laser beam path. Additional beams can also be added to the optical path.

Optical combiner for combining multiple laser beams in a flow cytometer

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Windsor, CO, United States of America

Kevin P Raley