Xenogen Corporation

Caliper Life Sciences, Inc.

Kevin P Francis

Kevin A Nawotka

Timothy C Doyle

Pamela R Contag

Rajendra Singh

Ning Zhang

Anthony Purchio

Daniel Ansaldi

Danny J Joh

Compositions and methods are described for detecting enzyme activity in a live organism (e.g., animal) are provided.

In vivo imaging of enzymatic activity

The present invention provides methods of introducing a polynucleotide into a target cell, wherein the method employs a light generating protein coding sequence acting as a reporter. An important advantage of the methods described herein is that drug resistant target cells or target cells having no useful auxotrophic markers can be effectively transformed. The present invention also includes transformed cells produced by the methods described herein. Also described are light generating protein coding sequence modifications, a variety of vectors, and methods of using the transformed cells of the present invention.

Methods of screening for introduction of DNA into a target cell

The present invention relates to bacterial luciferase transposon cassettes suitable for conferring bioluminescence properties on a Gram-positive bacteria, Gram-negative bacteria, and other organisms of interest. The invention further includes cells transformed with vectors carrying the transposon cassettes, cells whose genomes have been modified by introduction of such cassettes, and methods of making and using such transposon cassettes, transposon cassette vectors, and cells containing the transposons.

Compositions and methods for use thereof in modifying the genomes of microorganisms

The present invention relates to bacterial luciferase expression cassettes suitable for conferring bioluminescence properties on Gram-positive bacteria, cells transformed with such cassettes, and methods of making and using such cassettes.

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Alameda, CA, United States of America

Kevin P Francis