E.i. Dupont De Nemours and Company

Other

Kevin Michael Croker

Qiong Cheng

Kristin Ruebling-Jass

Jianzhong Zhang

Hong Peng Wang

Kostantinos Kourtakis

Stephen R Fahnestock

Michael P Perry

Tanja Maria Gruber

Paul Douglas Stull

James M Prober

Shane Crawford Weber

Qi Chen

Seth Lawrence Taylor

Michael B Damore

Thomas Eduard Friedmann

Disrupting the expression of endogenoushost cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinanthost cells are provided as well as methods of using such host cells for heterologous peptide production.

Host cell modifications that improve peptide production and downstream processing

A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.

Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins

Disrupting the expression of endogenoushost cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production relative to controlhost cells. Recombinanthost cells are provided as well as methods of using such host cells for heterologous peptide production.

Gene knockout mutations that increase peptide production

A microsphere for use in a bioassay comprising a glass core coated with a nanoparticle composite comprising a bioactive probe is provided. The nanoparticle composite coating enhances the density of bioprobe loading on the surface of the microspheres, resulting in enhanced dynamic range and sensitivity in bioassays. The particle may be used in detection systems where resonant light scattering properties of the particle are useful.

Nanoparticle composite-coated glass microspheres for use in bioassays

Gel pads and gel pad arrays, and methods for making and using them, are disclosed. The gel pads preferably comprise an intelligent gel.

Gel pad arrays and methods and systems for making them

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Hockessin, DE, United States of America

Kevin Michael Croker