Pharmacia Corporation

Omegatech, Inc.

Kevin L Duffin

Carol Pearcy Howard

Dean James Welsch

Dawn R Dufield

David G Corley

James P Doom

Olga V Nemirovskiy

Teresa Sunyer

Samuel G Zeller

A method for identifying and quantifying peptides resulting from enzyme cleavage of collagen type II by mass spectrometric analysis to detect characteristic peptide fragments of a carboxy-terminus having known mass to charge ratios to identify the peptide having that carboxy-terminus. Identification and quantification of the peptides in a biological sample is used to assess activity of proteolytic enzymes in diseases or physiological conditions such as osteoarthritis and rheumatoid arthritis and to assess the efficacy of enzyme blocking agents.

Peptide biomarker and method of identification

This invention is directed to a process for separating a first triglyceride comprising a docosahexaenoic acid residue from a second triglyceride. The process comprises introducing a feed mixture comprising the first triglyceride and the second triglyceride into a chromatographic separation zone, and then isolating a fraction of the feed mixture in the separation zone that has a mass ratio of the first triglyceride to the second triglyceride that is greater than in the feed mixture. The separation zone comprises a stationary phase which comprises metal ions that are capable of coordinating with a double bond of a fatty acid residue of the first triglyceride to form a metal complex with the fatty acid residue.

Process for separating a triglyceride comprising a docosahexaenoic acid residue from a mixture of triglycerides

Growing community of inventors

Manchester, MO, United States of America

Kevin L Duffin