Onyx Therapeutics, Inc.

System Biosciences, LLC

Kevin Kwei

Christopher J Kirk

Fangting Wu

Jeremiah Degenhardt

Brian Tuch

Andrea Loehr

Travis John Antes

Methods of treating a tumor in a subject and methods of determining a treatment regimen for a subject with a tumor are provided herein. In exemplary aspects, the methods comprise measuring the level of expression of immunoglobulin, FCGR2B, a gene listed in Table 4, or a combination thereof. In exemplary aspects, the subject is a subject from which a sample was obtained, wherein the level of immunoglobulin, FCGR2B, a gene listed in Table 4, or a combination thereof, has been measured from the sample. Related kits, computer readable-storage media, systems, and methods implemented by a processor in a computer are further provided.

Immunoglobulin expression levels as biomarker for proteasome inhibitor response

The invention relates to compositions and methods for isolation of microvesicles produced by mammalian cells. These microvesicles, known as extracellular microvesicles or circulating microvesicles, are isolated from sample materials such as body fluids, or from cell culture media that has been used to culture and maintain mammalian cells in vitro. The isolation of microvesicles as described herein results in purification and concentration of the microvesicles. The invention also provides related methods for producing blood serum and/or blood plasma that is free of detectable microvesicles, largely depleted of microvesicles, or has reduced concentration of microvesicles compared to the blood serum or blood plasma starting material (collectively termed 'microvesicle-depleted'). The generation of microvesicle-depleted blood serum or plasma is critical for use in experimental systems where it is desirable to use a cell culture media that does not contain endogenous microvesicles, or has been depleted of endogenous microvesicles, from the source material.

Methods for microvesicle isolation and selective removal

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Mountain View, CA, United States of America

Kevin Kwei