University of Texas System

Kevin H Gardner

Carlos A Amezcua

Steven L McKnight

Carolyn Michnoff

Laura B Motta-Mena

Shannon Harper

Jared Rutter

Charles G Caldwell

Doug E Frantz

Richard Keith Bruick

John B MacMillan

Uttam K Tambar

Thomas H Scheuermann

Brian D Zotowski

Brian D Zoltowski

Paulus J A Erbel

Paul B Card

The present invention provides methods for light-dependent gene regulation using a light-responsive DNA-binding protein. Also provided are related nucleic acid molecules, and protein molecules, such as those encoding or comprising the light-responsive DNA-binding protein or DNA-binding sites recognizing the light-responsive DNA-binding protein. Kits using the present light-dependent gene regulation system are further provided by the present invention.

Blue light-inducible system for gene expression

Provided is a method of inhibiting heterodimerization of HIF-2&#x3b1; to HIF1&#x3b2; (ARNT) comprising binding certain small molecules to the HIF-2&#x3b1; PAS-B domain cavity but not to HIF1&#x3b1; and inhibiting HIF-2&#x3b1; heterodimerization to HIF1&#x3b2; (ARNT) but not inhibiting HIF1&#x3b1; heterodimerization to HIF1&#x3b2; (ARNT). Those certain small molecules are also referenced synonymously as HIF2-HDI and HIF2&#x3b1; heterodimerization inhibitors and also simply as certain small molecules.

Inhibition of HIF-2&#x3b1; heterodimerization with HIF1&#x3b2; (ARNT)

Blue-light inducible system for gene expression

Specific binding of a foreign core ligand to a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is determined using NMR spectra of the PAS domain in the presence and absence of a foreign ligand bound within the hydrophobic core. A functional surface binding specificity of a PAS domain is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.

NMR detection of foreign PAS domain ligands

The invention provides methods and compositions relating to a novel kinase designated PAS Kinase (PASK). The compositions include isolated polypeptides comprising a native PASK protein or a PASK N-terminal domain and polypeptides consisting of a PASK PAS-A or PAS-B domain, as well as isolated polynucleotides encoding such polypeptides, and expression vectors and cells comprising such polynucleotides. The methods include binding assays comprising the steps of incubating a mixture comprising a subject polypeptide with a ligand under conditions wherein the polypeptide binds the ligand; and detecting binding of the polypeptide to the ligand.

PAS kinase

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Dallas, TX, United States of America

Kevin H Gardner