E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Corteva Agriscience LLC

Kevin G Ripp

Knut Meyer

Howard Glenn Damude

Kevin L Stecca

Anthony John Kinney

William Dean Hitz

Edgar Benjamin Cahoon

John D Everard

Scott H Diehn

Lynne Eileen Sims

Shreedharan Sriram

Cory M Larsen

Holly Jean Butler

Bryce Reid Daines

Geny I Anthony

Lyudmila Sidorenko

Gilda M Rauscher

Bo Shen

Thomas G Patterson

Rebecca E Cahoon

Quinn Qun Zhu

Zhan-Bin Liu

Zhan-bin Liu

Thomas J Carlson

Hyeon-Je Cho

Hyeon-je Cho

Bryce Daines

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmCAB2 gene. Some embodiments relate to a promoter or a 5' UTR from a GmCAB2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a GmCAB2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Plant promoter for transgene expression

3'UTR sequence for transgene expression

Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.

Leghemoglobin in soybean

Recombinant DNA constructs comprising the soybean sucrose synthase promoter operably linked to polynucleotides encoding transcription factors such as ODP1, Lec1 and FUSCA3 are disclosed. These constructs are used for increasing oil content while maintaining normal germination in oilseed plants. Methods to increase oil content in the seeds of an oilseed plant using this construct are also disclosed herein.

Use of the soybean sucrose synthase promoter to increase plant seed lipid content

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmCAB2 gene. Some embodiments relate to a promoter or a 5' UTR from a GmCAB2 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3&#x2032; UTR or a terminator from a GmCAB2 gene that functions in plants to promote transcription of operably linked nucleotide sequences.

Recombinant DNA constructs comprise plastidic carbonic anhydrase coding sequences operably linked to polynucleotides encoding DGAT or transcription factors such as ODP1. These constructs can be used to increase oil content in plants and seeds to levels that are not achieved with DGAT alone. Methods of generating plants containing the constructs and for increasing oil content in the seeds of an oilseed plant are also disclosed.

Plastidic carbonic anhydrase genes for oil augmentation in seeds with increased DGAT expression

Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.

DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

A recombinant DNA construct comprising a polynucleotide encoding an ODP1 polypeptide operably linked to a sucrose synthase 2 promoter where this construct can be used to increase oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination is disclosed. A method for increasing oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination using this construct is also disclosed.

Use of a seed specific promoter to drive ODP1 expression in cruciferous oilseed plants to increase oil content while maintaining normal germination

Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these multizymes in plants and oleaginous yeast are disclosed.

Multizymes comprising delta-9 elongase and delta-8 desaturase and their use in making polyunsaturated fatty acids

Growing community of inventors

Wilmington, DE, United States of America

Kevin G Ripp