Illumina Corporation

Cue Health Inc.

Kevin Eric Wu

Chen Zhao

John P Taylor

Raphael Shapiro

Clint Sever

Han-Yu Chuang

Alex So

Michael Credelle

Randall Hill

Omar Fontes

Jay Greenwood

Stephen Tanner

Stephen M Gross

Dwight Baker

Tatjana Singer

Sven Bilke

Jennifer Lococo

Disclosed herein are systems and methods for collapsing sequencing reads and identifying similar sequencing reads. In one example, a method includes generating a plurality of first identifier subsequences from a first identifier sequence of each nucleotide sequencing read and generating a first signature for the nucleotide sequencing read by applying hashing to the plurality of first identifier subsequences. The method may include assigning the nucleotide sequencing read to a first particular bin of a first data structure based on the first signature and determining a nucleotide sequence for each first particular bin of the first data structure with one or more nucleotide sequencing reads assigned.

Systems and methods for grouping and collapsing sequencing reads

Basal attachment for thermometer

Ear attachment for thermometer

Thermometer

The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Universal short adapters with variable length non-random unique molecular identifiers

The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the unique molecular index sequences include variable-length nonrandom sequences. In some implementations, the unique molecular index sequences are associated with the individual polynucleotide fragments based on alignment scores indicating similarity between the unique molecular index sequences and subsequences of sequence reads obtained from the individual polynucleotide fragments. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths

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Oceanside, CA, United States of America

Kevin Eric Wu