Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Kevin D Loutherback

J Tanner Nevill

Jian Gong

John A Tenney

Alexander J Mastroianni

Aathavan Karunakaran

Randall D Lowe, Jr

Kevin T Chapman

I-Jong Lin

Troy A Lionberger

Gang F Wang

Xiaohua Wang

Gregory G Lavieu

Peter J Beemiller

Yelena Bronevetsky

Shao Ning Pei

Natalie C Marks

Guido K Stadler

Matthew E Fowler

Duane Smith

Amanda L Goodsell

Annamaria Mocciaro

Anupam Singhal

Phillip J M Elms

Jr Randall D Lowe

Andrew W Mcfarland

Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (D) that separates activated T lymphocytes from na&#xef;ve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from na&#xef;ve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

Sorting of T lymphocytes in a microfluidic device

Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

Methods, systems and kits for in-pen assays

Growing community of inventors

Emeryville, CA, United States of America

Kevin D Loutherback