New York Stem Cell Foundation, Inc.

Q-State Biosciences, Inc.

Harvard College

The General Hospital Corporation

Children's Medical Center Corporation

Kevin C Eggan

Scott Noggle

Adam Ezra Cohen

Stephen Chang

Joel Kralj

Evangelos Kiskinis

Susan L Solomon

Clifford J Woolf

Brian J Wainger

Dietrich M Egli

Christoph Bock

Griet Annie Frans Verstappen

Alexander Meissner

Adam Cohen

The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.

Automated system for producing induced pluripotent stem cells or differentiated cells

The invention generally relates to optical methods for the diagnosis of neuronal condition by converting a cell from a patient into a neuron and optically evaluating action potentials of that cell in vitro. The cell is transformed with an optical reporter and exhibits an optical signature in response to neural stimulation. Using genome-editing, a control cell can be made that is isogenic but-for a known mutation and a control signature obtained from the control cell. Thus, methods of the invention reveal potential neurodegenerative effects of a mutation as manifested in a patient's genetic context. The optical signature of the cell, or the difference between the signature and the control signature, is correlated to a diagnosis of the neurodegenerative disease.

Diagnostic methods for neural disorders

The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells. The invention system is useful for isolating somatic cells from tissue samples, producing iPSC lines from adult differentiated cells by reprogramming such cells, identifying the pluripotent reprogrammed adult cells among other cells, and expanding and screening the identified reprogrammed cells.

Methods are provided for producing a human embryo capable of developing to the blastocyst stage. The method includes transferring a human somatic cell genome into a mature human oocyte by nuclear transfer and activating the oocyte, without removing the oocyte genome. Pluripotent human embryonic stem cells, and methods of obtaining these, are also provided.

Method for producing pluripotent stem cells

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Boston, MA, United States of America

Kevin C Eggan