The University of British Columbia

Kevin Albert Heyries

Daniel Jay Da Costa

Marketa Ricicova

Carl Lars Genghis Hansen

Michael Andrew Vaninsberghe

Georgia Elizabeth Mewis

Anupam Singhal

Carl L G Hansen

Veronique Lecault

Hans Zahn

Ester Falconer

Oleh Petriv

Brad Nelson

Julie Nielsen

Kathleen Lisaingo

Carl Lars Hansen

Carl L Hansen

Provided herein are high-throughput sequencing methods to study the diversity and functionality of lymphocyte receptor chains and pairing of the same. Specifically, the methods provided herein are used to identify with confidence one or more lymphocyte receptor chain pairs in a sample, for example one or more functional chain pairs.

Methods for determining lymphocyte receptor chain pairs

Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

Microfluidic devices and methods for use thereof in multicellular assays of secretion

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Vancouver, Canada

Kevin Albert Heyries