Medizinische Hochschule Hannover

Other

Biomimetics Technologies, Inc.

Schmälzlin, Elmar

Stolz, Marvin

Kerstin Reimers

Peter Vogt

Christina Allmeling

Meir Israelowitz

Herbert Peter Von Schroeder

Christoph Gille

Birgit Weyand

Elmar Schmälzlin

Elmar Schmaelzlin

The invention provides a process for producing a three-dimensional tissue by cultivating eucaryotic cells by introducing the cells into a matrix and cultivating the cells within the matrix in a cell culture medium within a cell culture vessel under controlled dissolved oxygen conditions of the cell culture medium. The matrix contains a support containing an optical oxygen sensor, which is an oxygen-sensitive dye, which upon irradiation with an excitation wavelength changes its emission characteristics in dependence on the dissolved oxygen concentration in the surrounding medium, including a dye phosphorescing upon irradiation of an excitation wavelength, which phosphorescence is quenched by dissolved oxygen.

Process for producing three-dimensional tissue

The present invention provides the use of spider silk threads for the production of tissue implants for medical use. A scaffolding matrix is provided for implantation, which matrix is comprised of spider silk thread and/or segments of spider silk threads which are cross-linked. The cross-linked spider silk thread segments essentially maintain a three-dimensional shape into which it is formed by pressing between molds. The three-dimensional shape obtainable by molding sufficiently stable to be used as an implant or for colonizing by mammalian cells while essentially maintaining the three-dimensional shape. In a further embodiment, the scaffolding matrix of cross-linked spider silk segments is at least partially covered by cells autologous or heterologous to the implant recipient. The matrix colonized with cells is obtainable by in vitro cultivation of isolated cells in suspension with the scaffolding matrix.

Implant of cross-linked spider silk threads

The invention relates to a biocompatible neural implant for bridging interruptions or defects in nerves resulting from injuries sustained in an accident or following surgery. Inventive neural implants comprise fibers made from natural or synthetic spider silk and allow interrupted nerves to regenerate across defects such that functional nerve conduction is made possible within a short period of time.

Neural implant

Growing community of inventors

Hannover, Germany

Kerstin Reimers