Bayer Technology Services Gmbh

Siemens Healthcare Diagnostics Gmbh

Centrum Fur Angewandte Nanotechnologie (can) Gmbh

Kerstin Bohmann

Werner Hoheisel

Burkhard Köhler

Guido Hennig

Markus Haase

Christiane Meyer

Ralph Markus Wirtz

Ingmar Dorn

Heike Euting

Thorsten Heidelberg

Stephan Schwers

Birge Schopper

Stefan Haubold

Elke Reifenberger

Stephan c/o Hanosolutions Gmbh Haubold

The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

Method for filtering nucleic acids, in particular from fixed tissue

Luminescent inorganic nanoparticles comprising: (a) a core made from a first metal salt or oxide being surrounded by (b) a shell made from a second metal salt or oxide being luminescent and having non-semiconductor properties. These nanoparticles can be advantageously used in (fluorescence) resonance energy transfer ((F)RET)-based bioassays in view of their higher (F)RET efficiency.

Luminescent core/shell nanoparticles suitable for (F)RET-assays

The present invention relates to an automatable method for the improved diagnosis of pathologically altered cells by simultaneously staining at least two different molecular markers, which exhibit a change in gene expression which is disease-associated, in a cell or constituent regions of a tissue sample by means of using combinations of antibodies and accrediting signal intensities.

Method for increasing clinical specificity when detecting tumors and their precursor stages by simultaneously measuring at least two different molecular markers

The invention relates to an assay based on resonance energy transfer (RET), comprising a first molecule grope A, which is marked with at least one energy donor, and at least one second molecule group B which is marked with at least one energy acceptor, the donor comprising a molecule or particle which can be energetically excited by an external radiation source and which is fluorescence enabled and the acceptor comprising a molecule or particle which can be excited by energy transfer via the donor with partial or complete quenching of the donor fluorescence, and the donor and/or acceptor comprise luminescing inorganic dope nanoparticles having an expansion of &#x2266;50 nanometers, emitting electromagnetic radiation with stokes or anti-stokes scattering after energetic excitation.

Assay based on doped nanoparticles

A process for synthesizing nanoparticles, in particular metal salt nanoparticles. To the synthesis mixture is added a modifying reagent which binds, by means of a first functional group, to the nanoparticle surface and which carries a second functional group for binding to molecules which are specifically selected in dependence on the subsequent use of the nanoparticles. This dispenses with a postsynthetic, separate, application-specific modification step. A new substance class, the pentaalkyl iminobis(methylenephosphono)carboxylates, are particularly suitable for this purpose. These modifying reagents permit the nanoparticles to grow in a specifically controlled manner and, at the same time, modify the surface of the growing nanoparticles, (in-situ) during the synthesis, such that the particles can be very readily dissolved in a large number of solvents and carry functional groups for coupling on molecules, resulting in the particles having, immediately after having been synthesized, a certain all-round usability.

Production and use of in situ-modified nanoparticles

Growing community of inventors

Kö'ln, Germany

Kerstin Bohmann